Early Weaning Inhibits Intestinal Stem Cell Expansion to Disrupt the Intestinal Integrity of Duroc Piglets via Regulating the Keap1/Nrf2 Signaling.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ying-Chao Qin, Cheng-Long Jin, Ting-Cai Hu, Jia-Yi Zhou, Xiao-Fan Wang, Xiu-Qi Wang, Xiang-Feng Kong, Hui-Chao Yan
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Abstract

There are different stress resistance among different breeds of pigs. Changes in intestinal stem cells (ISCs) are still unclear among various breeds of piglets after early weaning. In the current study, Taoyuan Black and Duroc piglets were slaughtered at 21 days of age (early weaning day) and 24 days of age (3 days after early weaning) for 10 piglets in each group. The results showed that the rate of ISC-driven epithelial renewal in local Taoyuan Black pigs hardly changed after weaning for 3 days. However, weaning stress significantly reduced the weight of the duodenum and jejunum in Duroc piglets. Meanwhile, the jejunal villus height, tight junction-related proteins (ZO-1, Occludin, and Claudin1), as well as the trans-epithelial electrical resistance (TEER) values, were down-regulated after weaning for 3 days in Duroc piglets. Moreover, compared with Unweaned Duroc piglets, the numbers of Olfm4+ ISC cells, PCNA+ mitotic cells, SOX9+ secretory progenitor cells, and Villin+ absorptive cells in the jejunum were reduced significantly 3 days after weaning. And ex vivo jejunal crypt-derived organoids exhibited growth disadvantages in weaned Duroc piglets. Notably, the Keap1/Nrf2 signaling activities and the expression of HO-1 were significantly depressed in weaned Duroc piglets compared to Unweaned Duroc piglets. Thus, we can conclude that ISCs of Duroc piglets were more sensitive to weaning stress injury than Taoyuan Black piglets, and Keap1/Nrf2 signaling is involved in this process.

早期断奶通过调控 Keap1/Nrf2 信号抑制杜洛克仔猪肠干细胞扩增以破坏肠道完整性
不同品种的猪具有不同的抗应激能力。不同品种的仔猪在早期断奶后肠干细胞(ISCs)的变化仍不清楚。在本研究中,桃源黑猪和杜洛克仔猪分别在 21 日龄(早期断奶日)和 24 日龄(早期断奶后 3 天)屠宰,每组 10 头仔猪。结果表明,当地桃源黑猪在断奶 3 天后,ISC 驱动的上皮更新率几乎没有变化。然而,断奶应激明显降低了杜洛克仔猪十二指肠和空肠的重量。同时,断奶3天后,杜洛克仔猪的空肠绒毛高度、紧密连接相关蛋白(ZO-1、Occludin和Claudin1)以及跨上皮电阻(TEER)值均出现下调。此外,与未断奶杜洛克仔猪相比,断奶 3 天后空肠中 Olfm4+ ISC 细胞、PCNA+ 有丝分裂细胞、SOX9+ 分泌祖细胞和 Villin+ 吸收细胞的数量显著减少。断奶后的杜洛克仔猪体内空肠隐窝衍生的器官组织表现出生长劣势。值得注意的是,与未断奶杜洛克仔猪相比,断奶杜洛克仔猪的Keap1/Nrf2信号活性和HO-1的表达均显著降低。因此,我们可以得出结论:与桃源黑猪相比,杜洛克仔猪的ISC对断奶应激损伤更敏感,而Keap1/Nrf2信号转导参与了这一过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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