Different Oxidative Stress and Inflammation Patterns of Diseased Left Anterior Descending Coronary Artery versus Internal Thoracic Artery.

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2024-09-28 DOI:10.3390/antiox13101180

Andrea Salica, Vittoria Cammisotto, Raffaele Scaffa, Giulio Folino, Ruggero De Paulis, Roberto Carnevale, Umberto Benedetto, Wael Saade, Antonino Marullo, Sebastiano Sciarretta, Gianmarco Sarto, Silvia Palmerio, Valentina Valenti, Mariangela Peruzzi, Fabio Miraldi, Francesco Giosuè Irace, Giacomo Frati

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引用次数: 0

Abstract

Background: Oxidative stress and inflammation are typically implied in atherosclerosis pathogenesis and progression, especially in coronary artery disease (CAD). Our objective was to investigate the oxidative stress and inflammation burden directly associated with atherosclerotic plaque in patients with stable coronary disease undergoing coronary artery bypass graft (CABG) surgery. Specifically, markers of oxidative stress and inflammation were compared in blood samples obtained from the atherosclerotic left anterior descending artery (LAD) and blood samples obtained from the healthy left internal thoracic artery (LITA), used as a bypass graft, within the same patient.

Methods: Twenty patients scheduled for off-pump CABG were enrolled. Blood samples were collected from the LITA below anastomosis and the LAD below the stenosis. Samples were analysed for oxidative stress (sNOXdp, H₂O₂, NO) and inflammation markers (TNFα, IL-6, IL-1β, IL-10).

Results: The analysis showed a significant increase in oxidative stress burden in the LAD as compared to LITA, as indicated by higher sNOX2-dp and H₂O₂ levels and lower NO levels (p < 0.01). Also, pro-inflammatory cytokines were increased in the LAD as compared to the LITA, as indicated by higher TNFα and IL-6 amounts (p < 0.01). On the other hand, no significant differences could be seen regarding IL-1β and IL-10 levels between the two groups.

Conclusions: The oxidative stress and inflammatory burden are specifically enhanced in the LAD artery of stable coronary patients compared to systemic blood from the LITA of stable coronary patients.

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病变左前降支冠状动脉与胸内动脉的氧化应激和炎症模式不同

背景：氧化应激和炎症通常与动脉粥样硬化的发病和进展有关，尤其是在冠状动脉疾病（CAD）中。我们的目的是研究接受冠状动脉旁路移植（CABG）手术的稳定型冠心病患者体内与动脉粥样硬化斑块直接相关的氧化应激和炎症负担。具体来说，比较了同一患者从动脉粥样硬化的左前降支动脉（LAD）采集的血液样本和从健康的左胸内动脉（LITA）（用作旁路移植）采集的血液样本中的氧化应激和炎症指标：方法：20 名计划接受非泵 CABG 手术的患者入组。从吻合口下方的 LITA 和狭窄处下方的 LAD 采集血液样本。对样本进行氧化应激（sNOXdp、H2O2、NO）和炎症指标（TNFα、IL-6、IL-1β、IL-10）分析：分析表明，与LITA相比，LAD的氧化应激负荷明显增加，表现为sNOX2-dp和H2O2水平升高，NO水平降低（P < 0.01）。此外，与 LITA 相比，LAD 中的促炎细胞因子增加，表现为 TNFα 和 IL-6 水平升高（p < 0.01）。另一方面，两组间的IL-1β和IL-10水平无明显差异：结论：与来自稳定期冠心病患者 LITA 的全身血液相比，稳定期冠心病患者 LAD 动脉中的氧化应激和炎症负荷明显增加。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.