An Integrated Strategy for Establishing the Chemical Profile of Premna Microphylla Turcz. Leaves and Metabolites in Vivo.

Journal of AOAC International Pub Date : 2025-01-01 DOI:10.1093/jaoacint/qsae079

Jinhong Cai, Shenghong Guan, Xueli Hu, Xuezhao Chen, Xiaosun Liu, Shouxin Li, Jingkui Tian, Ping Wang, Hua Gu, Xiaoyong Zhang

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Abstract

Background: Premna microphylla Turcz. (PMT) is a traditional food and medicinal plant, which has been used to treat cure hemostasis, rheumatism, and dysentery. However, there is still a lack of a clear understanding of the chemical profile of PMT and its metabolites in vivo.

Objective: To establish a rapid and efficient analytical method for the identification of phytochemicals in PMT and their metabolites in vivo.

Methods: First, the fingerprint of PMT was established by HPLC with method validation. Then, the phytochemical composition of PMT leaves was identified using ultra-performance liquid chromatography-quadrupole time-of-flight-tandem mass spectrometry (UPLC-QTOF-MS/MS). Finally, the prototype and correlated metabolites were detected after oral administration in mice to understand the absorption and metabolism of phytochemicals in vivo.

Results: The results showed that the established HPLC method for fingerprint evaluation of PMT has good precision, repeatability, and stability. Additionally, a total of 103 phytochemicals were identified in PMT, including mainly flavonoids and terpenoids. Then, 37 prototype components and 20 derived metabolites in vivo were detected.

Conclusion: In this study, we constructed a fingerprint method, which has good stability, precision, and repeatability, and the fingerprint of PMT was established. Then, the chemical profile of PMT in vitro and in vivo was determined. The results showed that flavonoids and terpenoids were the main phytochemicals in PMT, and methylation, sulfonation, and dihydroxylation were the main metabolic pathway in vivo.

Highlights: The present study provides the phytochemical basis for subsequent study of pharmacological activity.

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用于建立 Premna Microphylla Turcz.

背景：Premna microphylla Turcz.(PMT) 是一种传统的食用和药用植物，被用于治疗止血、风湿和痢疾。然而，人们对其体内的化学成分和代谢产物仍缺乏清晰的认识：目的：建立一种快速、高效的分析方法，用于鉴定首乌藤中的植物化学成分及体内代谢产物：方法：首先，通过高效液相色谱法（HPLC）建立 PMT 的指纹图谱，并进行方法学验证。然后，利用超高效液相色谱-串联四重飞行时间质谱（UPLC-QTOF-MS/MS）对 PMT 叶片中的植物化学成分进行鉴定。最后，在小鼠口服后检测其原型和相关代谢物，以了解植物化学物质在体内的吸收和代谢情况：结果表明，所建立的高效液相色谱法具有良好的精密度、重复性和稳定性。此外，共鉴定出 PMT 中的 103 种植物化学物质，主要包括黄酮类和萜类化合物。结论：本研究构建了一种具有良好稳定性、精确性和重复性的指纹图谱方法，并建立了 PMT 的指纹图谱。然后对 PMT 在体外和体内的化学特征进行了分析。结果表明，黄酮类和萜类化合物是 PMT 的主要植物化学成分，甲基化、磺化和二羟基化是其体内的主要代谢途径：本研究为后续的药理活性研究提供了植物化学基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of AOAC International

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