Lifelong Changes in the Choroidal Thickness, Refractive Status, and Ocular Dimensions in C57BL/6J Mouse.

IF 5 2区 医学 Q1 OPHTHALMOLOGY
Tao Tang, Chi Ren, Yi Cai, Yan Li, Kai Wang, Mingwei Zhao
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引用次数: 0

Abstract

Purpose: To investigate the changes in choroidal thickness (ChT), refractive status, and ocular dimensions in the mouse eye in vivo using updated techniques and instrumentation.

Methods: High-resolution swept-source optical coherence tomography (SS-OCT), eccentric infrared photoretinoscopy, and custom real-time optical coherence tomography were used to analyze choroidal changes, refractive changes and ocular growth in C57BL/6J mice from postnatal day (P) 21 to month 22.

Results: The ChT gradually increased with age, with the thickest region in the para-optic nerve head and thinning outward, and the temporal ChT was globally thicker than the nasal ChT. Retinal thickness remained stable until 4 months and subsequently decreased. The average spherical equivalent refraction error was -4.81 ± 2.71 diopters (D) at P21, which developed into emmetropia by P32, reached a hyperopic peak (+5.75 ± 1.38 D) at P82 and returned to +0.66 ± 1.86 D at 22 months. Central corneal thickness, anterior chamber depth, lens thickness, and axial length (AL) increased continuously before 4 months, but subsequently exhibited subtle changes. Vitreous chamber depth decreased with lens growth. ChT was correlated significantly with the ocular parameters (except for retinal thickness) before the age of 4 months, but these correlations diminished after 4 months. Furthermore, for mice younger than 4 months, the difference in the ChT, especially temporal ChT, between the two eyes contributed most to that of axial length and spherical equivalent refraction error.

Conclusions: Four months could be a watershed age in the growth of mouse eyes. Large-span temporal recordings of refraction, ocular dimensions, and choroidal changes provided references for the study of the physiological and pathological mechanisms responsible for myopia.

C57BL/6J 小鼠脉络膜厚度、屈光状态和眼部尺寸的终生变化
目的:使用最新技术和仪器研究小鼠体内脉络膜厚度(ChT)、屈光状态和眼球尺寸的变化。方法:使用高分辨率扫源光学相干断层扫描(SS-OCT)、偏心红外光视网膜镜和定制实时光学相干断层扫描分析C57BL/6J小鼠从出生后第21天到第22个月的脉络膜变化、屈光变化和眼球生长:随着年龄的增长,视网膜厚度逐渐增加,视神经头旁区域最厚,并向外变薄,颞侧视网膜厚度总体上比鼻侧视网膜厚度厚。视网膜厚度在 4 个月前保持稳定,随后有所下降。P21时的平均球面等效屈光度误差为-4.81 ± 2.71屈光度(D),P32时发展为散光,P82时达到远视峰值(+5.75 ± 1.38屈光度),22个月时恢复到+0.66 ± 1.86屈光度。中央角膜厚度、前房深度、晶状体厚度和轴长(AL)在 4 个月前持续增加,但随后出现了微妙的变化。玻璃体腔深度随着晶状体的增长而减小。4 个月前,ChT 与眼部参数(视网膜厚度除外)有明显的相关性,但 4 个月后这些相关性减弱。此外,对于小于4个月的小鼠,两眼ChT的差异,尤其是颞侧ChT的差异,对轴向长度和球面等效屈光误差的影响最大:结论:4 个月可能是小鼠眼睛生长的分水岭。对屈光度、眼球尺寸和脉络膜变化的大跨度时间记录为研究近视的生理和病理机制提供了参考。
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来源期刊
CiteScore
6.90
自引率
4.50%
发文量
339
审稿时长
1 months
期刊介绍: Investigative Ophthalmology & Visual Science (IOVS), published as ready online, is a peer-reviewed academic journal of the Association for Research in Vision and Ophthalmology (ARVO). IOVS features original research, mostly pertaining to clinical and laboratory ophthalmology and vision research in general.
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