Interference patterns of two-photon excited fluorescence by spatial beam shaping

IF 3.1 3区物理与天体物理 Q2 Engineering

Optik Pub Date : 2024-09-28 DOI:10.1016/j.ijleo.2024.172054

R. Gottschalk, A. Lindinger

引用次数: 0

Abstract

We report on two-photon excited fluorescence interference patterns produced by spatial laser beam shaping. Thereto, a focused spatially modulated laser beam component is overlapped with an unfocused Gaussian beam component, and both are directed on a cuvette filled with dye. The phase retardance between the two common path laser components is precisely adjusted by a 2D liquid crystal modulator. With this method it is possible to generate various fluorescence patterns which exhibit sharp features and a high contrast due to constructive and destructive interference of a two-photon excitation process. The developed spatial shaping interference technique with two-photon excitations has a high potential for optical and biophotonic imaging applications.

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通过空间光束整形实现双光子激发荧光的干涉模式

我们报告了空间激光光束整形产生的双光子激发荧光干涉图案。为此，一个聚焦的空间调制激光束分量与一个非聚焦的高斯光束分量重叠，两者都对准一个装满染料的比色皿。两个共同路径激光分量之间的相位延迟可通过二维液晶调制器进行精确调节。利用这种方法可以产生各种荧光图案，由于双光子激发过程的建设性和破坏性干涉，这些图案表现出鲜明的特征和高对比度。所开发的双光子激发空间塑形干涉技术在光学和生物光子成像应用方面具有很大的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optik 物理-光学

CiteScore

6.90

自引率

12.90%

发文量

1471

审稿时长

46 days

期刊介绍： Optik publishes articles on all subjects related to light and electron optics and offers a survey on the state of research and technical development within the following fields: Optics: -Optics design, geometrical and beam optics, wave optics- Optical and micro-optical components, diffractive optics, devices and systems- Photoelectric and optoelectronic devices- Optical properties of materials, nonlinear optics, wave propagation and transmission in homogeneous and inhomogeneous materials- Information optics, image formation and processing, holographic techniques, microscopes and spectrometer techniques, and image analysis- Optical testing and measuring techniques- Optical communication and computing- Physiological optics- As well as other related topics.