Novel mechanistic understanding that Lactiplantibacillus plantarum is more capable of improving the ensiling performance of wheat straw silage than xylanase by driving certain key metabolites

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2024-10-12 DOI:10.1186/s40538-024-00677-8

Haoran Yu, Richa Hu, Yushan Jia, Yanzi Xiao, Shuai Du

{"title":"Novel mechanistic understanding that Lactiplantibacillus plantarum is more capable of improving the ensiling performance of wheat straw silage than xylanase by driving certain key metabolites","authors":"Haoran Yu, Richa Hu, Yushan Jia, Yanzi Xiao, Shuai Du","doi":"10.1186/s40538-024-00677-8","DOIUrl":null,"url":null,"abstract":"<div><p>Microbial and enzyme additives can improve silage performance, but there is limited comparative research on the effects of microbial and enzyme additives on improving silage fermentation quality, and the underlying microbial and metabolic pathways remain unclear. This study investigated the effects without inoculants (CK treatment) or with <i>Lactiplantibacillus plantarum</i> (LP treatment)<i>,</i> xylanase (XY treatment) and their combination (LPXY treatment) on the fermentation quality, as well as on the microbial communities and metabolite profiles of the wheat straw silage. The results demonstrated that the LP treatment has a better effect on improving the fermentation quality of wheat straw silage compared to other treatments, as evidenced by markedly (<i>p</i> < 0.05) decreased the pH (4.06), acid and neutral fiber (ANF, NDF, 23.43 and 31.69%DM), and increased the lactic acid (LA, 965.89 mg/L) and acetic acid (AA, 656.10 mg/L) concentrations. After the fermentation process, the LP treatment significantly (<i>p</i> < 0.05) enhanced the abundance of <i>Lactobacillus</i>, reduced bacterial Shannon (<i>p</i> < 0.05) and increased some key metabolites content. The structural equation models (SEMs) and Pearson’s correlation results proved that the LP treatment improved the wheat straw silage fermentation quality via increasing the abundance of <i>Lactobacillus</i>, decreasing the diversity of bacterial community and enriching the content of certain key metabolites. The present study provides mechanistic evidence that <i>Lactiplantibacillus plantarum</i> additive is superior to xylanase additive and their combination on improving fermentation quality of wheat straw silage, that is, by enriching certain key metabolites to increase AA and LA concentrations, providing a reference for the cross study of silage feed fermentation microbiome and metabolome.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":512,"journal":{"name":"Chemical and Biological Technologies in Agriculture","volume":"11 1","pages":""},"PeriodicalIF":5.2000,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chembioagro.springeropen.com/counter/pdf/10.1186/s40538-024-00677-8","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical and Biological Technologies in Agriculture","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1186/s40538-024-00677-8","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Microbial and enzyme additives can improve silage performance, but there is limited comparative research on the effects of microbial and enzyme additives on improving silage fermentation quality, and the underlying microbial and metabolic pathways remain unclear. This study investigated the effects without inoculants (CK treatment) or with Lactiplantibacillus plantarum (LP treatment), xylanase (XY treatment) and their combination (LPXY treatment) on the fermentation quality, as well as on the microbial communities and metabolite profiles of the wheat straw silage. The results demonstrated that the LP treatment has a better effect on improving the fermentation quality of wheat straw silage compared to other treatments, as evidenced by markedly (p < 0.05) decreased the pH (4.06), acid and neutral fiber (ANF, NDF, 23.43 and 31.69%DM), and increased the lactic acid (LA, 965.89 mg/L) and acetic acid (AA, 656.10 mg/L) concentrations. After the fermentation process, the LP treatment significantly (p < 0.05) enhanced the abundance of Lactobacillus, reduced bacterial Shannon (p < 0.05) and increased some key metabolites content. The structural equation models (SEMs) and Pearson’s correlation results proved that the LP treatment improved the wheat straw silage fermentation quality via increasing the abundance of Lactobacillus, decreasing the diversity of bacterial community and enriching the content of certain key metabolites. The present study provides mechanistic evidence that Lactiplantibacillus plantarum additive is superior to xylanase additive and their combination on improving fermentation quality of wheat straw silage, that is, by enriching certain key metabolites to increase AA and LA concentrations, providing a reference for the cross study of silage feed fermentation microbiome and metabolome.

Graphical Abstract

查看原文本刊更多论文

从新的机制角度认识植物乳杆菌比木聚糖酶更能通过驱动某些关键代谢物改善小麦秸秆青贮的腐熟性能

微生物和酶添加剂可提高青贮饲料的性能，但有关微生物和酶添加剂对提高青贮饲料发酵质量的影响的比较研究有限，其潜在的微生物和代谢途径仍不清楚。本研究调查了不添加接种剂（CK 处理）或添加植物乳杆菌（LP 处理）、木聚糖酶（XY 处理）及其组合（LPXY 处理）对小麦秸秆青贮发酵质量以及微生物群落和代谢物谱的影响。结果表明，与其他处理相比，LP处理对改善小麦秸秆青贮的发酵质量有更好的效果，具体表现为pH值（4.06）、酸性纤维和中性纤维（ANF、NDF，23.43%和31.69%DM）明显降低（p < 0.05），乳酸（LA，965.89 mg/L）和乙酸（AA，656.10 mg/L）浓度升高。发酵过程结束后，LP 处理显著提高了乳酸菌的丰度（p < 0.05），减少了细菌香农（p < 0.05），并增加了一些关键代谢物的含量。结构方程模型（SEMs）和皮尔逊相关性结果证明，LP 处理通过提高乳酸杆菌的丰度、降低细菌群落的多样性和丰富某些关键代谢物的含量来改善小麦秸秆青贮发酵质量。本研究提供了植物乳杆菌添加剂在改善小麦秸秆青贮发酵质量方面优于木聚糖酶添加剂及其组合的机理证据，即通过富集某些关键代谢产物来提高AA和LA的浓度，为青贮饲料发酵微生物组和代谢组的交叉研究提供了参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.