Hydrogen Sulfide and 5-Aminolevulinic Acid Synergistically Enhance Drought Tolerance in Tomato (Solanum lycopersicum L.)

IF 4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Energy Security Pub Date : 2024-10-08 DOI:10.1002/fes3.70007

Cengiz Kaya, Ferhat Uğurlar

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Abstract

Enhancing crop drought tolerance is crucial for food security amid climate change. This study examines how 5-aminolevulinic acid (ALA) and hydrogen sulfide (H₂S) can improve drought resilience in tomato plants, which are essential for sustainable food production. Drought stress was induced using 12% PEG-6000. Plants were pre-treated with 25 mg L⁻¹ ALA and 0.1 mg L⁻¹ hypotaurine (HT), followed by 0.2 mM sodium hydrosulfide (NaHS) treatment to assess the effects on plant physiological effects over 10 days. Drought stress reduced plant dry weight, chlorophylls (a and b), F_v/F_m, leaf water potential, and relative water content, while increasing glycine betaine (GB) and proline levels. Additionally, drought stress elevated NADPH oxidase (NOX) and glycolate oxidase (GOX) activities, inducing oxidative stress and membrane damage. ALA and NaHS enhanced plant growth, photosynthesis, proline, GB, ALA content, ATP synthase, and ATPase activities, while mitigating NOX and GOX activities, thereby reducing $O_{2}^{\cdot -}$ and H₂O₂ radicals. ALA alone boosted L-DES activity, promoting H₂S accumulation. However, ALA + HT reduced H₂S levels, compromising ALA's efficacy. NaHS with ALA + HT reinstated positive effects by restoring H₂S levels. Biochemical assays confirmed ALA and NaHS promoted H₂S accumulation, bolstering antioxidants, mitigating lipid peroxidation, suggesting their drought resilience potential in tomatoes.

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硫化氢和 5-Aminolevulinic Acid 协同增强番茄（Solanum lycopersicum L.）的抗旱能力

提高作物的抗旱能力对于气候变化下的粮食安全至关重要。本研究探讨了 5-氨基乙酰丙酸（ALA）和硫化氢（H2S）如何提高番茄植株的抗旱能力，这对可持续粮食生产至关重要。使用 12% PEG-6000 诱导干旱胁迫。用 25 mg L-1 ALA 和 0.1 mg L-1 低牛磺酸（HT）对植物进行预处理，然后用 0.2 mM 硫氢化钠（NaHS）处理，以评估 10 天内对植物生理效应的影响。干旱胁迫降低了植物干重、叶绿素（a 和 b）、Fv/Fm、叶片水势和相对含水量，同时增加了甘氨酸甜菜碱（GB）和脯氨酸水平。此外，干旱胁迫提高了 NADPH 氧化酶（NOX）和乙醇酸氧化酶（GOX）的活性，诱发氧化胁迫和膜损伤。ALA 和 NaHS 提高了植物的生长、光合作用、脯氨酸、GB、ALA 含量、ATP 合酶和 ATP 酶活性，同时减轻了 NOX 和 GOX 活性，从而减少了 O 2 - $$ {mathrm{O}}_2^{\cdotp -}$ 和 H2O2 自由基。单独使用 ALA 会增强 L-DES 的活性，促进 H2S 的积累。然而，ALA + HT 降低了 H2S 水平，从而削弱了 ALA 的功效。使用 ALA + HT 的 NaHS 恢复了 H2S 水平，从而恢复了积极效果。生化试验证实，ALA 和 NaHS 可促进 H2S 积累，增强抗氧化剂，减轻脂质过氧化反应，这表明它们在番茄中具有抗旱潜力。

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来源期刊

Food and Energy Security Energy-Renewable Energy, Sustainability and the Environment

CiteScore

9.30

自引率

4.00%

发文量

审稿时长

19 weeks

期刊介绍： Food and Energy Security seeks to publish high quality and high impact original research on agricultural crop and forest productivity to improve food and energy security. It actively seeks submissions from emerging countries with expanding agricultural research communities. Papers from China, other parts of Asia, India and South America are particularly welcome. The Editorial Board, headed by Editor-in-Chief Professor Martin Parry, is determined to make FES the leading publication in its sector and will be aiming for a top-ranking impact factor. Primary research articles should report hypothesis driven investigations that provide new insights into mechanisms and processes that determine productivity and properties for exploitation. Review articles are welcome but they must be critical in approach and provide particularly novel and far reaching insights. Food and Energy Security offers authors a forum for the discussion of the most important advances in this field and promotes an integrative approach of scientific disciplines. Papers must contribute substantially to the advancement of knowledge. Examples of areas covered in Food and Energy Security include: • Agronomy • Biotechnological Approaches • Breeding & Genetics • Climate Change • Quality and Composition • Food Crops and Bioenergy Feedstocks • Developmental, Physiology and Biochemistry • Functional Genomics • Molecular Biology • Pest and Disease Management • Post Harvest Biology • Soil Science • Systems Biology