The Acid-Stimulated Self-Assembled DNA Nanonetwork for Sensitive Detection and Living Cancer Cell Imaging of MicroRNA-221.

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2024-10-11 DOI:10.1021/acs.analchem.4c03055

Yichen Han, Mengshi Jiang, Jie Zhou, Hongmin Lei, Ruo Yuan, Yaqin Chai

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引用次数: 0

Abstract

Herein, a novel functional DNA structure, acid-stimulated self-assembly DNA nanonetwork (ASDN), was proposed for miRNA-221 sensitive detection and high-resolution living cancer cell imaging. Significantly, the self-assembly of ASDN only occurred in the acidic extracellular environment of cancer cells, which could be endocytosed by cancer cells to eliminate the interference of noncancer cells and deliver the ASDN into cancer cells. Subsequently, endogenous miRNA-221 could trigger the catalytic hairpin assembly within ASDN, resulting in the separation of the fluorophore Cy5 and the quencher BHQ2 to recover the substantial Cy5 fluorescence signals, thus achieving signal amplification for sensitive detection of miRNA-221 with a detection limit of 5.5 pM, as well as facilitating high-resolution and low-background imaging of miRNA-221 in cancer cells. In consequence, this strategy provides an innovative DNA nanonetwork to distinguish cancer cells from other cells for sensitive detection of biomarkers, offering a meaningful reference for the application of DNA nanostructure self-assembly technology in relevant fundamental research and disease diagnosis.

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酸刺激自组装 DNA 纳米网络用于 MicroRNA-221 的灵敏检测和活体癌细胞成像。

本文提出了一种新型功能DNA结构--酸刺激自组装DNA纳米网络（ASDN），用于miRNA-221的灵敏检测和高分辨率活体癌细胞成像。值得注意的是，ASDN的自组装只发生在癌细胞的酸性胞外环境中，它可以被癌细胞内吞，排除非癌细胞的干扰，将ASDN送入癌细胞。随后，内源性 miRNA-221 可触发 ASDN 内的催化发夹组装，导致荧光团 Cy5 和淬灭剂 BHQ2 分离，恢复大量的 Cy5 荧光信号，从而实现信号放大，以 5.5 pM 的检测限灵敏检测 miRNA-221，并促进癌细胞中 miRNA-221 的高分辨率、低背景成像。因此，该策略提供了一种创新的 DNA 纳米网络，用于区分癌细胞和其他细胞，从而实现生物标记物的灵敏检测，为 DNA 纳米结构自组装技术在相关基础研究和疾病诊断中的应用提供了有意义的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.