Analysis of the life cycle of Helicobacter pylori bacteriophage KHP40 belonging to the genus Schmidvirus.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae082

Masahiro Iwamoto, Michiko Takahashi, Hiromichi Maeda, Hiroaki Takeuchi, Jumpei Uchiyama, Takako Ujihara, Keizo Nagasaki, Kazuhiro Hanazaki, Satoru Seo, Naoya Kitamura, Tetsuya Yamamoto, Shigenobu Matsuzaki

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引用次数: 0

Abstract

Bacteriophage (phage) KHP40 was previously isolated from the supernatant of a culture of Helicobacter pylori KMT83 cells. In this study, we analysed the infection characteristics of KHP40, phage release pattern from KMT83 cells, and state of KHP40 DNA in KMT83 cells. The findings revealed that KHP40 phage showed varied adsorption efficiencies for different strains, long latent periods, and small burst sizes. Additionally, KHP40 activity was maintained at pH 2.5-12. KHP40 phages were released during the vegetative growth phase of the KMT83 cells. PCR analysis demonstrated that KHP40 DNA was stably maintained in KMT83 clones. Next-generation sequencing analysis revealed the presence of two distinct types of circular double-stranded DNA in H. pylori KMT83 cells. One was an H. pylori-specific DNA consisting of 1 578 403 bp, and the other was a 26 412-bp sequence that represented the episomal form of phage KHP40 DNA. Furthermore, defective KHP40-lysogenic DNA was detected in the H. pylori-specific DNA, the deleted portion of which appeared to have been transferred to another location in the bacterial genome. These findings indicate that KHP40 DNA exists in both episomal and defectively lysogenized states in KMT83 cells, and active phages are produced from KHP40-episomal DNA.

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幽门螺杆菌噬菌体 KHP40（属于施米德病毒属）的生命周期分析。

此前，我们从幽门螺杆菌 KMT83 细胞培养物的上清液中分离出了噬菌体（噬菌体）KHP40。本研究分析了 KHP40 的感染特征、KMT83 细胞中噬菌体的释放模式以及 KMT83 细胞中 KHP40 DNA 的状态。研究结果表明，KHP40噬菌体对不同菌株的吸附效率不同，潜伏期长，爆发量小。此外，KHP40 的活性在 pH 值为 2.5-12 时仍能保持。KHP40 噬菌体在 KMT83 细胞的无性生长期释放。PCR分析表明，KMT83克隆中稳定地保留了KHP40 DNA。新一代测序分析显示，幽门螺杆菌 KMT83 细胞中存在两种不同类型的环状双链 DNA。一种是由 1,578,403 bp 组成的幽门螺杆菌特异性 DNA，另一种是 26,412 bp 序列，代表噬菌体 KHP40 DNA 的外显子形式。此外，在幽门螺杆菌特异性DNA中还检测到了有缺陷的KHP40裂殖DNA，其中被删除的部分似乎转移到了细菌基因组的另一个位置。这些发现表明，KMT83细胞中的KHP40 DNA既存在于表膜状态，也存在于缺陷裂解状态，活性噬菌体是由KHP40-表膜DNA产生的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.