Antifungal activity of poly(lactic acid) nanofibers containing the essential oil from Corymbia citriodora Hook or the monoterpenes β-citronellol and citronellal against mycotoxigenic fungi.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae083

Alex Rodrigues Silva Caetano, Maria das Graças Cardoso, Juliano Elvis de Oliveira, Luiz Roberto Batista, Eduardo Alves, Caio Vinicius Lima Natarelli, Gabriela Aguiar Campolina, Vanuzia Rodrigues Fernandes Ferreira, David Lee Nelson

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引用次数: 0

Abstract

Food contamination by mycotoxigenic fungi is one of the principal factors that cause food loss and economic losses in the food industry. The objective of this work was to incorporate the essential oil from Corymbia citriodora Hook and its constituents citronellal and β-citronellol into poly(lactic acid) nanofibers; to characterize the nanofibers by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy and differential scanning calorimetry; to evaluate the antifungal activity by the fumigation method; to evaluate the antimycotoxigenic activity against Aspergillus carbonarius, Aspergillus ochraceus, Aspergillus westerdijkiae, Aspergillus flavus, and Aspergillus parasiticus; and to evaluate the morphology of these microorganisms. All the nanofibers had a regular, smooth, and continuous morphology. FTIR analyses confirmed that the active ingredients were incorporated into the polymer matrix. All samples exhibited antifungal and ochratoxigenic inhibitory activities of up to 100% and 99%, respectively, with the best results observed for (PLA + 30 wt% β-citronellol) nanofibers and (PLA + 30 wt% citronellal) nanofibers. However, 100% inhibition of the production of aflatoxin B1 and B2 was not observed. The images obtained by SEM indicated that the nanofibers caused damage to the hyphae, caused a decrease in the production of spores, and caused deformation, rupture, and non-formation of the conid head, might be an alternative for the control of mycotoxigenic fungi.

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含有 Corymbia citriodora Hook 精油或单萜烯 β-香茅醇和香茅醛的聚（乳酸）纳米纤维对霉菌毒素真菌的抗真菌活性。

霉菌毒素污染食品是造成食品损失和食品工业经济损失的主要因素之一。这项工作的目的是将 Corymbia citriodora Hook 的精油及其成分香茅醛和β-香茅醇加入聚乳酸纳米纤维中；通过扫描电子显微镜（SEM）、傅立叶变换红外光谱（FTIR）和差示扫描量热法（DSC）对纳米纤维进行表征；通过熏蒸法评估纳米纤维的抗真菌活性；评估纳米纤维对碳曲霉、赭曲霉、西洋曲霉、黄曲霉和寄生曲霉的抗霉菌毒素活性；以及评估这些微生物的形态。所有纳米纤维都具有规则、光滑和连续的形态。傅立叶变换红外分析证实，活性成分已融入聚合物基质。所有样品的抗真菌和赭曲霉毒素抑制活性分别高达 100%和 99%，其中（聚乳酸 + 30 wt% β-香茅醇）纳米纤维和（聚乳酸 + 30 wt% 香茅醛）纳米纤维的效果最好。然而，并未观察到对黄曲霉毒素 B1 和 B2 的产生有 100% 的抑制作用。扫描电子显微镜获得的图像表明，纳米纤维对菌丝造成了破坏，导致孢子产量减少，并使分生孢子头部变形、破裂和不变形，可能是控制霉菌毒素真菌的一种替代方法。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.