Signal switching electrochemical and fluorescence dual-mode sensing platform for carbendazim determination based on “two-in-one” magneto-fluorescent Cdots

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2024-09-30 DOI:10.1016/j.foodchem.2024.141494

Yuzhen Jia , Yuanmeng Ke , Zhixiang Liu , Huaixia Yang , Mingsan Miao , Liang Guo

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引用次数: 0

Abstract

An innovative method for carbendazim (CBZ) detection was developed, consisting of an electrochemical-fluorescence dual-mode biosensor based on magneto-fluorescent composite M-CDs. M-CDs, as the fluorescent probe of this sensor, could combine the electrical signal-ferrocene to achieve the “signal switching” by specifically recognizing CBZ through aptamers, of which magnetic property was used to quickly separate from complex substrates without interference. The dual-mode sensor based on M-CDs demonstrated excellent linear responses in both electrochemical and fluorescence assays. It achieved detection ranges of 10 fg/mL − 300 ng/mL and 60 fg/mL – 100 ng/mL with detection limits (LODs) of 1.4 fg/mL and 2.3 fg/mL. The sensor exhibited exceptional detection performance, stability and anti-interference. In addition, the results of the sensor in actual samples were consistent with those of enzyme-linked immunosorbent assay (ELISA), which further demonstrated that the sensor could accurately trace detecting CBZ in real samples and had a certain application prospect.

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基于 "二合一 "磁性荧光 Cdots 的多菌灵测定信号切换电化学和荧光双模传感平台

基于磁荧光复合 M-CDs 的电化学-荧光双模式生物传感器是一种检测多菌灵（CBZ）的创新方法。M-CDs 作为该传感器的荧光探针，可以将电信号与二茂铁结合起来，通过适配体特异性识别 CBZ 来实现 "信号切换"，而适配体的磁性可以不受干扰地从复杂基质中快速分离出来。基于 M-CDs 的双模式传感器在电化学和荧光检测中都表现出了出色的线性响应。它的检测范围为 10 fg/mL - 300 ng/mL 和 60 fg/mL - 100 ng/mL，检测限（LOD）分别为 1.4 fg/mL 和 2.3 fg/mL。该传感器具有优异的检测性能、稳定性和抗干扰性。此外，该传感器在实际样品中的检测结果与酶联免疫吸附试验（ELISA）的结果一致，进一步证明了该传感器能准确痕量检测实际样品中的 CBZ，具有一定的应用前景。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.