The Combination of Host-Associated Bacillus megaterium R32 and Stachyose Promotes the Intestinal Health of Turbot (Scophthalmus maximus. L)

IF 3 2区农林科学 Q1 FISHERIES

Aquaculture Nutrition Pub Date : 2024-09-30 DOI:10.1155/2024/8658386

Yaoyao Kong, Sifan Zhao, Weihao Ou, Kangsen Mai, Yanjiao Zhang

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Abstract

An 8-week feeding trial was conducted to investigate the effects of host-associated Bacillus megaterium R32 and stachyose on the intestinal mucosal defense system of turbot (Scophthalmus maximus. L). Three isonitrogenous and isolipidic diets were formulated: control diet (C), control diet with 1.0 × 10⁸ CFU/g B. megaterium R32 (RC), and 1.0 × 10⁸ CFU/g B. megaterium R32 + 1.5% stachyose (RS) supplementation separately. The results showed that diets RS and RC significantly inhibited the expression of cell development and apoptosis-related genes (β-catenin, CyclinD1, BAX, Bid); diets RS and RC significantly increased the expression of intestinal tight junction protein claudin-4, while RS group significantly decreased the expression of myosin light chain kinase; diets RS and RC significantly decreased the expression of proinflammatory factors (IL-13, IL-15, IFN- γ), diet RS also significantly decreased the expression of TNF-α and AP-1, and increased the expression of TGF-β. 16s rRNA gene sequencing results showed that diets RS and RC significantly decreased the abundance of conditional pathogenic bacteria (Corynebacterium, Desulfovibrio, Escherichia-Shigella). Among them, the abundance of Bacillus in the RS group was the highest. It is concluded that the combination of stachyose and B. megaterium R32 had a more positive effect on intestinal cell development and barrier function and strengthened the intestinal mucosal defense system of turbot.

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与宿主相关的巨型芽孢杆菌 R32 和水苏糖结合使用可促进大菱鲆的肠道健康

进行了一项为期 8 周的饲养试验，以研究宿主相关的巨型芽孢杆菌 R32 和水苏糖对大菱鲆（Scophthalmus maximus.L）肠道粘膜防御系统的影响。分别配制了三种等氮隔脂日粮：对照日粮（C）、添加 1.0 × 108 CFU/g 巨型芽孢杆菌 R32 的对照日粮（RC）和添加 1.0 × 108 CFU/g 巨型芽孢杆菌 R32 + 1.5% 水苏糖的日粮（RS）。结果表明，RS和RC显著抑制细胞发育和凋亡相关基因（β-catenin、CyclinD1、BAX、Bid）的表达；RS和RC显著增加肠紧密连接蛋白claudin-4的表达，而RS组则显著降低肌球蛋白轻链激酶的表达；RS和RC日粮明显降低了促炎因子（IL-13、IL-15、IFN- γ）的表达，RS日粮还明显降低了TNF-α和AP-1的表达，提高了TGF-β的表达。16s rRNA基因测序结果表明，日粮RS和RC显著降低了条件致病菌（棒状杆菌、脱硫弧菌、埃希氏-志贺氏菌）的数量。其中，RS 组的芽孢杆菌数量最多。结论是水苏糖和巨型芽孢杆菌 R32 的组合对大菱鲆肠道细胞发育和屏障功能有更积极的影响，并能增强大菱鲆的肠道粘膜防御系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture Nutrition 农林科学-渔业

CiteScore

7.20

自引率

8.60%

发文量

131

审稿时长

3 months

期刊介绍： Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers. Aquaculture Nutrition publishes papers which strive to: increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research. improve understanding of the relationships between nutrition and the environmental impact of aquaculture. increase understanding of the relationships between nutrition and processing, product quality, and the consumer. help aquaculturalists improve their management and understanding of the complex discipline of nutrition. help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.