Ultrasensitive Analysis of Escherichia coli O157:H7 Based on Immunomagnetic Separation and Labeled Surface-Enhanced Raman Scattering with Minimized False Positive Identifications

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2024-09-27 DOI:10.1021/acs.jafc.4c06311

Yang Zhang, Chuping Zhao, Zhiming Guo, Tianxi Yang, Xinai Zhang, Xiaowei Huang, Jiyong Shi, Shipeng Gao, Xiaobo Zou

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Abstract

It is a big challenge to monitor pathogens in food with high selectivity. In this study, we reported an ultrasensitive method for Escherichia coli O157:H7 detection based on immunomagnetic separation and labeled surface-enhanced Raman scattering (SERS). The bacterium was identified by heterogeneous recognition elements, monoclonal antibody (mAb), and aptamer. E. coli O157:H7 was separated and enriched by magnetic nanoparticles modified by mAb, and then a plasmonic nanostructure functionalized by aptamers with embedded Raman tags and interior gaps was utilized for further discrimination and detection. The selectivity was enhanced by two binding sites. The higher Raman enhancement was obtained by strong local electromagnetic field oscillation in the gap and the firm embedment of 4-mercaptopyridine (4-Mpy). Optimum experiments created that SERS signals of 4-Mpy at 1010 cm^–1 had a good linearity with E. coli O157:H7 at a large range of 10 to 10⁷ CFU/mL with a limit of detection of 2 CFU/mL. This method has great potential for on-site food pathogenic bacterial detection.

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基于免疫磁性分离和标记表面增强拉曼散射的大肠埃希氏菌 O157:H7 超灵敏分析，将假阳性识别率降至最低

如何高选择性地监测食品中的病原体是一项巨大的挑战。在这项研究中，我们报道了一种基于免疫磁性分离和标记表面增强拉曼散射（SERS）的超灵敏大肠杆菌 O157:H7 检测方法。该方法通过异质识别元件、单克隆抗体（mAb）和适配体对大肠杆菌进行了鉴定。利用经 mAb 修饰的磁性纳米粒子对大肠杆菌 O157:H7 进行了分离和富集，然后利用内嵌拉曼标签和内部间隙的灵敏剂功能化的质子纳米结构进行了进一步的鉴别和检测。两个结合位点增强了选择性。间隙中强烈的局部电磁场振荡和 4-巯基吡啶（4-Mpy）的牢固嵌入获得了更高的拉曼增强。最佳实验结果表明，4-Mpy 在 1010 cm-1 处的 SERS 信号与大肠杆菌 O157:H7 在 10 至 107 CFU/mL 的大范围内具有良好的线性关系，检测限为 2 CFU/mL。该方法在现场食品致病菌检测方面具有很大的潜力。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.