Biocontrol of Colletotrichum fructicola in the Postharvest Banana Fruit Using the Siderophore-Producing Strain BX1

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2024-09-24 DOI:10.1021/acs.jafc.4c04726

Yiming Zhu, Erxun Zhou, Canwei Shu, Baoping Cheng, Xiaoxue Liu, Xiaolin Tang, Lingtao Duan, Chongjian Ma, Jianye Chen, Wangjin Lu, Yingying Yang

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Abstract

Banana anthracnose, caused by Colletotrichum fructicola, significantly reduced the postharvest fruit quality. Employing biocontrol strategies offers a sustainable approach to enhance agricultural practices. The Burkholderia sp. strain BX1 hinders the growth and appressorium formation of C. fructicola, and its sterile filtrate lowers the anthracnose incidence while preserving the fruit quality. Scanning electron microscopy and genomic analyses confirmed BX1 as Burkholderia pyrrocinia. AntiSMASH analysis identified three siderophores with high similarity, and improved MALDI-TOF IMS confirmed the presence of the siderophore pyochelin. Furthermore, the BX1 filtrate suppressed the expression of virulence genes in C. fructicola and induced the expression of disease resistance genes in banana. However, the presence of 80 μM iron ions notably mitigated BX1’s inhibitory effects and reversed the changes in related gene expression. These results underscore BX1’s robust efficacy as a biocontrol agent in managing banana anthracnose, highlight the effective antifungal compounds, and elucidate the influence of environmental factors on biocontrol effectiveness.

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利用产苷酸菌株 BX1 生物防治采后香蕉果实中的果胶串珠菌

香蕉炭疽病由果孢子菌（Colletotrichum fructicola）引起，大大降低了采后水果的质量。采用生物防治策略为改善农业生产提供了一种可持续的方法。伯克霍尔德氏菌 BX1 菌株能阻碍果核孢子菌的生长和附着体的形成，其无菌滤液能降低炭疽病的发病率，同时保持果实的品质。扫描电子显微镜和基因组分析证实 BX1 是伯克霍尔德氏吡咯霉素菌。AntiSMASH 分析确定了三种具有高度相似性的嗜酸性物质，改进的 MALDI-TOF IMS 证实了嗜酸性物质 pyochelin 的存在。此外，BX1滤液抑制了果蝇毒力基因的表达，并诱导了香蕉抗病基因的表达。然而，80 μM 铁离子的存在明显减轻了 BX1 的抑制作用，并逆转了相关基因表达的变化。这些结果突出了 BX1 作为生物防治剂在防治香蕉炭疽病方面的强大功效，强调了有效的抗真菌化合物，并阐明了环境因素对生物防治效果的影响。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.