Alkaline electrolyzed water-assisted ultrasonic extraction of lutein from daylily (Hemerocallis spp.): Structural preservation and activity evaluation

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-09-18 DOI:10.1016/j.foodchem.2024.141334

Yifu Zhang , Tiantian Zhang , Tongliang Yang , Xixian Xie , Ye Chen

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引用次数: 0

Abstract

During lutein extraction, isomerization from all-trans to cis form reduced stability and antioxidant capacity. Alkaline electrolyzed water (AEW), with its reduced-state and small molecular clusters, effectively extracted bioactive substances and inhibited oxidation. Thus, an AEW-assisted ultrasonic extraction method was used to extract lutein from daylily. Compared to ultrasonic extraction alone, AEW treatment increased the lutein yield by 60 %. Structural analysis confirmed the preservation of all-trans lutein by AEW. Assess AEW extraction's impact on lutein's bioactivity by examining its protection against blue light cell damage. The results showed that that lutein extracted using AEW exhibited enhanced antioxidant capacity, significantly boosting the viability of ARPE-19 cells and the activity of intracellular antioxidant enzymes, thereby mitigating oxidative stress damage to the retina caused by blue light exposure. This study provided an effective method for efficiently extracting bioactive substances and preventing the impact of the extraction process on their structure and function.

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碱性电解水辅助超声波提取萱草叶黄素：结构保存和活性评估

在叶黄素提取过程中，从全反式到顺式的异构化降低了叶黄素的稳定性和抗氧化能力。碱性电解水（AEW）具有还原态和小分子团，能有效提取生物活性物质并抑制氧化。因此，我们采用了 AEW 辅助超声波萃取法来提取萱草中的叶黄素。与单独进行超声波提取相比，AEW 处理使叶黄素的产量提高了 60%。结构分析证实，AEW 可保留全反式叶黄素。通过检测叶黄素对蓝光细胞损伤的保护作用，评估 AEW 提取对叶黄素生物活性的影响。结果表明，使用 AEW 提取的叶黄素具有更强的抗氧化能力，能显著提高 ARPE-19 细胞的活力和细胞内抗氧化酶的活性，从而减轻蓝光照射对视网膜造成的氧化应激损伤。这项研究为高效提取生物活性物质并防止提取过程对其结构和功能的影响提供了一种有效的方法。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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