Improving Vaccine Response through Probiotics and Micronutrient Supplementation: Evaluating the Role of TLR5 in Adult Female BALB/c Mice.

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Current pharmaceutical design Pub Date : 2024-09-10 DOI:10.2174/0113816128310203240823053538

Zohre Eftekhari, Delaram Doroud, Maryam Tajabadi-Ebrahimi, Fatemeh Kazemi-Lomedasht

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引用次数: 0

Abstract

Background: The role of probiotics and micronutrients in improving immune system function and response to vaccination has been proven. Hence, this study aimed to investigate the effects of probiotics enriched with micronutrients on the immunogenicity of PastoCovac® vaccine.

Methods: The probiotic supplement BioBoost® and PastoCovac® vaccine, which contain six expressed receptor-binding domains (RBD) and conjugated with tetanus toxin, were administered concurrently. The safety and efficacy were assessed by determining Immunoglobulin G (IgG) antibody titers to RBD and cytokines, mRNA expression of Toll-like Receptors (TLRs) 5, and clinical symptoms.

Results: Results revealed that the administration of the probiotics enriched with micronutrients and vitamins for 14 days before the first vaccine dose, followed by continued supplementation for 14 days after the first dose, and in conjunction with the second vaccine dose, yielded the most significant elevation in interleukin 4 (IL-4), Tumor Necrosis Factor-alpha (TNF alpha), Interferon-gamma (IFN-gamma), and anti-SARS-CoV-2 RBD IgG levels within the supernatant samples collected from spleen cultures with the highest expression of TLR5 genes in intestinal samples, compared to the control group.

Conclusion: Our results indicated that the inclusion of probiotics enriched with micronutrients and vitamins significantly enhanced the immunogenicity of the PastoCovac® vaccine. Based on the recommendation to administer third and fourth vaccine doses, particularly for vulnerable and elderly individuals, the utilization of supplements containing probiotics is expected to favorably influence immune responses.

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通过补充益生菌和微量营养素改善疫苗反应：评估TLR5在成年雌性BALB/c小鼠中的作用

背景：益生菌和微量营养素在改善免疫系统功能和疫苗接种反应方面的作用已得到证实。因此，本研究旨在探讨富含微量营养素的益生菌对PastoCovac®疫苗免疫原性的影响：方法：同时接种益生菌补充剂 BioBoost® 和 PastoCovac® 疫苗，后者含有六个表达的受体结合域 (RBD)，并与破伤风毒素共轭。通过测定 RBD 和细胞因子的免疫球蛋白 G (IgG) 抗体滴度、Toll 样受体 (TLR) 5 的 mRNA 表达以及临床症状来评估疫苗的安全性和有效性：结果：结果显示，在接种第一剂疫苗前 14 天服用富含微量元素和维生素的益生菌，在接种第一剂疫苗后 14 天继续补充，并在接种第二剂疫苗的同时服用富含微量元素和维生素的益生菌、与对照组相比，从脾脏培养物收集的上清样本中白细胞介素 4 (IL-4)、肿瘤坏死因子-α (TNF alpha)、γ 干扰素 (IFN-gamma) 和抗 SARS-CoV-2 RBD IgG 水平的升高最为明显，肠道样本中 TLR5 基因的表达量最高。结论我们的研究结果表明，加入富含微量营养素和维生素的益生菌可显著增强 PastoCovac® 疫苗的免疫原性。根据接种第三和第四剂疫苗的建议，尤其是针对易感人群和老年人，使用含有益生菌的补充剂有望对免疫反应产生有利影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current pharmaceutical design 医学-药学

CiteScore

6.30

自引率

0.00%

发文量

302

审稿时长

2 months

期刊介绍： Current Pharmaceutical Design publishes timely in-depth reviews and research articles from leading pharmaceutical researchers in the field, covering all aspects of current research in rational drug design. Each issue is devoted to a single major therapeutic area guest edited by an acknowledged authority in the field. Each thematic issue of Current Pharmaceutical Design covers all subject areas of major importance to modern drug design including: medicinal chemistry, pharmacology, drug targets and disease mechanism.