Heat-induced interactions between microfluidized hemp protein particles and caseins or whey proteins.

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2025-01-15 Epub Date: 2024-09-17 DOI:10.1016/j.foodchem.2024.141290

Sihan Ma, Aiqian Ye, Harjinder Singh, Alejandra Acevedo-Fani

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引用次数: 0

Abstract

The rising demand for sustainable proteins leads to increased interest in plant proteins like hemp protein (HP). However, commercial HP's poor functionality, including heat aggregation, limit its use. This study explored the heat-induced interactions of hemp protein particles (HPPs) with milk proteins, specifically whey proteins and caseins. Using various analysis techniques-static light scattering, TEM, SDS electrophoresis, surface hydrophobicity, and free sulfhydryl content-results showed that co-heating HPPs with whey protein isolate (WPI) or sodium caseinate (NaCN) at 95 °C for 20 min reduced HPPs aggregation. HPPs/WPI particles had a d_4,3 of ∼3.8 μm, while HPPs/NaCN were ∼1.9 μm, compared to ∼27.5 μm for HPPs alone. SDS-PAGE indicated that whey proteins irreversibly bound to HPPs, through disulfide bonds, whereas casein bound reversibly, possibly involving the chaperone-like property of casein. This study proposes possible mechanisms by which HPPs interact with milk proteins and impact protein aggregation. This may provide opportunities for developing hybrid protein microparticles.

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微流控大麻蛋白颗粒与酪蛋白或乳清蛋白之间的热诱导相互作用。

由于对可持续蛋白质的需求不断增加，人们对大麻蛋白（HP）等植物蛋白越来越感兴趣。然而，商业大麻蛋白的功能性较差，包括热聚集，限制了其使用。本研究探讨了大麻蛋白颗粒（HPPs）与牛奶蛋白（尤其是乳清蛋白和酪蛋白）在热诱导下的相互作用。利用各种分析技术--静态光散射、TEM、SDS 电泳、表面疏水性和游离巯基含量--结果表明，将大麻蛋白颗粒与乳清蛋白分离物（WPI）或酪蛋白酸钠（NaCN）在 95 °C 下共同加热 20 分钟可减少大麻蛋白颗粒的聚集。HPPs/WPI 颗粒的 d4,3 为 3.8 μm，而 HPPs/NaCN 为 1.9 μm，相比之下，单独使用 HPPs 时的 d4,3 为 27.5 μm。SDS-PAGE显示，乳清蛋白通过二硫键与HPPs不可逆地结合，而酪蛋白则可逆地结合，这可能与酪蛋白的类似伴侣的特性有关。这项研究提出了 HPP 与牛奶蛋白质相互作用并影响蛋白质聚集的可能机制。这可能为开发混合蛋白质微粒提供了机会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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