Microbial catabolism of coffee pulp (poly)phenols during in vitro colonic fermentation

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Biomaterials Science & Engineering Pub Date : 2024-09-18 DOI:10.1016/j.foodchem.2024.141354

Silvia Cañas , Nicole Tosi , Vanesa Núñez-Gómez , Daniele Del Rio , Pedro Mena , María A. Martín-Cabrejas , Yolanda Aguilera

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Abstract

Coffee pulp is a by-product characterized by its richness in phenolic compounds. This study examined the catabolism of (poly)phenols in digested coffee pulp flour (CPF) and extract (CPE) during in vitro colonic fermentation. After a simulated gastrointestinal digestion, samples were fermented using human microbiota and (poly)phenol transformations were analyzed by UHPLC-ESI-MS/MS. Digested CPF and CPE contained high amounts of phenolic acids, notably 3′,4′-dihydroxycinnamic (99.7–240.1 μmol 100 g⁻¹) and 3,4-dihydroxybenzoic acid (174.1–491.4 μmol 100 g⁻¹). During the in vitro fecal fermentation, phenylpropanoic acids (1.5- to 2.6-fold), phenyl-γ-valerolactones (1.3- to 23-fold), phenylvaleric acids (1.1- to 2-fold) and benzene derivatives (1.5-fold) increased; while benzoic and cinnamic acids, cinnamoylquinic derivatives, flavonols, benzaldehydes and diphenylpropan-2-ols decreased. The (poly)phenols in CPF were catabolized more slowly than in CPE, suggesting protection of the fibrous matrix against phenolic degradation. Coffee pulp may be a promising food ingredient rich in (poly)phenols contributing to the prevention of intestinal diseases.

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咖啡浆（多）酚在体外结肠发酵过程中的微生物分解作用

咖啡浆是一种副产品，其特点是富含酚类化合物。本研究考察了消化咖啡浆粉（CPF）和提取物（CPE）在体外结肠发酵过程中的（多）酚分解情况。在模拟胃肠道消化后，利用人体微生物群对样品进行发酵，并通过超高效液相色谱-电喷雾离子质谱/质谱分析（多）酚的转化。消化后的 CPF 和 CPE 含有大量酚酸，尤其是 3′,4′-二羟基肉桂酸（99.7-240.1 μmol 100 g-1）和 3,4- 二羟基苯甲酸（174.1-491.4 μmol 100 g-1）。在体外粪便发酵过程中，苯丙酸（1.5-2.6 倍）、苯基-γ-戊内酯（1.3-23 倍）、苯基戊酸（1.1-2 倍）和苯衍生物（1.5 倍）增加；而苯甲酸和肉桂酸、肉桂酰奎宁酸衍生物、黄酮醇、苯甲醛和二苯基丙-2-醇减少。与 CPE 相比，CPF 中的（多）酚分解速度更慢，这表明纤维基质具有防止酚类降解的保护作用。咖啡浆可能是一种富含（多）酚的有前途的食品配料，有助于预防肠道疾病。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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