Mapping the candidate gene controlling male sterility using EMS-mutagenized mutant smms1 in eggplant (Solanum melongena L.)

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2024-09-21 DOI:10.1016/j.scienta.2024.113622

Shuangxia Luo , Qiang Li , Yifan Kang , Yuhang Liu , Xing Wang , Ping Yu , Jiajia Shen , Lijun Song , Zhishuo Zhang , Xueping Chen

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引用次数: 0

Abstract

Male sterility (MS) is a desirable tool for hybrid seed production. Eggplant is one of the most widely grown fruit vegetables all over the world. Although a lot of male-sterile lines were created in eggplant, the genes underlying the MS regulation in eggplant remain largely unknown. EMS (ethyl methane sulfonate)- mutagenized mutant is an ideal material for the identification of genes regulating MS in eggplant. In the present study, we identified a mutant, named smms1 (Solanum melongena male sterility 1) with smaller flowers and abnormal anthers. Further analyses indicated the pollens in smms1 showed highly vacuolated and significant decreased number, size and viability compared to wild type (WT). Bulked segregant analysis sequencing (BSA-seq) and Mutmap analyses identified a G-to-A mutation in an alternative splicing region in the 2nd intron of Smechr0501647. Smechr0501647 encodes a subunit of Mediator (MED) that functions as a bridge between transcription factors and RNA polymerase II. The SNP results in three splicing events with 11 bp and 15 bp deletion in the 2nd exon and the retention of the 1st intron. Notably, the events with 11 bp deletion and intron retention led to premature stop codons. Gene Ontology (GO) enrichment of Differentially expressed gene (DEG) suggested that while up-regulated DEGs in smms1 are enriched in phytohormone related processes, the enriched GO terms of down-regulated DEGs were mainly related to cellular processes. The measurement of the phytohormone contents in WT and smms1 indicated the involvement of the abscisic acid (ABA), indole-3-acetic acid (IAA), salicylic acid (SA), jasmonic acid (JA) and cytokinins (CKs) in the MS regulation mediated by SmMS1 in eggplant. The discovery of the candidate gene controlling MS not only provides a potential target for the molecular breeding of MS germplasms, but also provides an effective basis of for studying regulatory mechanisms of MS in eggplant.

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利用茄子（Solanum melongena L.）的 EMS 诱变突变体 smms1，绘制控制雄性不育的候选基因图谱

雄性不育（MS）是杂交种子生产的理想工具。茄子是全世界种植最广泛的果菜之一。虽然在茄子中培育出了许多雄性不育系，但茄子中调控雄性不育的基因在很大程度上仍然未知。EMS（甲烷磺酸乙酯）诱变突变体是鉴定茄子 MS 调控基因的理想材料。在本研究中，我们发现了一个花朵较小且花药异常的突变体，命名为 smms1（Solanum melongena male sterility 1）。进一步的分析表明，与野生型（WT）相比，smms1 的花粉高度空泡化，数量、大小和活力显著下降。大量分离分析测序（BSA-seq）和突变图（Mutmap）分析确定了 Smechr0501647 第 2 个内含子中一个替代剪接区的 G 到 A 突变。Smechr0501647 编码 Mediator (MED) 的一个亚基，该亚基在转录因子和 RNA 聚合酶 II 之间起桥梁作用。该 SNP 导致三个剪接事件，其中第 2 个外显子缺失 11 bp 和 15 bp，第 1 个内含子保留。值得注意的是，11 bp 的缺失和内含子的保留会导致过早的终止密码子。差异表达基因（DEG）的基因本体（GO）富集表明，smms1 中上调的 DEGs 富集于与植物激素相关的过程，而下调的 DEGs 富集的 GO 术语主要与细胞过程有关。对 WT 和 smms1 植物激素含量的测定表明，脱落酸（ABA）、吲哚-3-乙酸（IAA）、水杨酸（SA）、茉莉酸（JA）和细胞分裂素（CKs）参与了 SmMS1 对茄子 MS 的调控。控制MS的候选基因的发现不仅为MS种质的分子育种提供了潜在的目标，也为研究茄子MS的调控机制提供了有效的依据。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.