Supplementation with cysteine improved metabolic syndrome in rats by increasing antioxidant potential in the liver and adipose tissue, as well as decreasing hepatic NF-κB expression

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Sina Mahdavifard , Manouchehr Nakhjavani
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引用次数: 0

Abstract

Insulin resistance is a key characteristic of metabolic syndrome (MetS). The hepatic nuclear factor- κB (NF-κB) signaling pathway plays a crucial role in insulin resistance and the development of type 2 diabetes. Our study aimed to examine the impact of cysteine (Cys) on various biochemical and histopathological parameters in the liver and kidney, hepatic NF-kβ expression, oxidative stress, inflammation, glycation, carbonyl stress markers, and insulin resistance. The study involved four groups of rats, each consisting of seven rats: a control group, a MetS group, and two similar groups receiving Cys treatment. Metabolic syndrome was induced in rats by administering a 40% sucrose solution, while, the treated groups received 50 mg/L Cys in their drinking water. Various factors, including body weight, hepatic NF-kβ expression, levels of antioxidants, anti-glycation, oxidative stress, carbonyl stress, inflammatory, anti-glycation, and glycation markers were assessed in blood and tissues. Liver and kidney function parameters and metabolic profiles were measured. Finally, liver tissue was also evaluated by a pathologist. The results showed that Cys reduced hepatic NF-kβ expression, oxidative stress, inflammation, glycation and carbonyl stress markers, as well as liver fatty content, blood sugar levels, insulin resistance, cardiovascular risk index, and body weight. The treatment also mitigated histopathological liver changes and acute hepatitis (p < 0.001). Cysteine exhibited anti-obesity and anti-atherosclerotic effects, improved β-cell function, insulin sensitivity, and lipid metabolism, and enhanced liver and kidney function, as well as prevented acute hepatitis by restoring the GSH/GSSG ratio, hepatic NF-kβ signaling, and carbonyl stress.

补充半胱氨酸可提高肝脏和脂肪组织的抗氧化潜力,减少肝脏 NF-κB 的表达,从而改善大鼠的代谢综合征
胰岛素抵抗是代谢综合征(MetS)的一个主要特征。肝脏核因子κB(NF-κB)信号通路在胰岛素抵抗和2型糖尿病的发展中起着至关重要的作用。我们的研究旨在探讨半胱氨酸(Cys)对肝脏和肾脏的各种生化和组织病理学参数、肝脏 NF-kβ 表达、氧化应激、炎症、糖化、羰基应激标记物和胰岛素抵抗的影响。研究涉及四组大鼠,每组七只:对照组、代谢综合征组和接受 Cys 治疗的两个类似组。通过给大鼠注射 40% 的蔗糖溶液诱发代谢综合征,而治疗组则在饮用水中添加 50 毫克/升的 Cys。对血液和组织中的各种因素进行了评估,包括体重、肝脏 NF-kβ 表达、抗氧化剂水平、抗糖化、氧化应激、羰基应激、炎症、抗糖化和糖化标志物。此外,还测量了肝肾功能参数和代谢概况。最后,病理学家还对肝组织进行了评估。结果显示,Cys 可降低肝脏 NF-kβ 表达、氧化应激、炎症、糖化和羰基应激标记物,以及肝脏脂肪含量、血糖水平、胰岛素抵抗、心血管风险指数和体重。治疗还能减轻肝脏组织病理学变化和急性肝炎(p < 0.001)。半胱氨酸具有抗肥胖和抗动脉粥样硬化的作用,可改善β细胞功能、胰岛素敏感性和脂质代谢,增强肝肾功能,并通过恢复GSH/GSSG比率、肝脏NF-kβ信号传导和羰基应激预防急性肝炎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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