Co-encapsulation of probiotic bacteria L. rhamnosus GG and β-carotene by a novel biphasic encapsulation technique: Stability and in vivo anti-inflammatory properties

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
{"title":"Co-encapsulation of probiotic bacteria L. rhamnosus GG and β-carotene by a novel biphasic encapsulation technique: Stability and in vivo anti-inflammatory properties","authors":"","doi":"10.1016/j.fbio.2024.105061","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, β-carotene (βC) was encapsulated in liposomes and spray-dried with the probiotic bacteria <em>Lactobacillus rhamnosus</em> GG (LGG), to obtain a biphasic structure with two functional components. Initially, the liposomes loaded with βC resulted in large multivesicular vesicles (LMVV) with spherical morphology, mean size of 1191 nm and entrapment efficiency of 81.33%. Then, the spray-drying of the mixture βC-LMVV with the LGG resulted in biphasic dried microparticles (BDM) with a spherical shape, retention of 64,48% of βC, and LGG survivor above 90%. The BDM showed high storage stability for 90 days at room temperature and, at the dose of 2000 mg/kg of BDM did not cause any acute toxicity in <em>Wistar</em> rats. In addition, at the same dose, presented significant anti-inflammatory activity in carrageenan-induced paw edema and pleurisy. Thus, the produced BDM could be an innovative ingredient with functional properties and also an efficient encapsulation strategy for βC.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Bioscience","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212429224014913","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

In this study, β-carotene (βC) was encapsulated in liposomes and spray-dried with the probiotic bacteria Lactobacillus rhamnosus GG (LGG), to obtain a biphasic structure with two functional components. Initially, the liposomes loaded with βC resulted in large multivesicular vesicles (LMVV) with spherical morphology, mean size of 1191 nm and entrapment efficiency of 81.33%. Then, the spray-drying of the mixture βC-LMVV with the LGG resulted in biphasic dried microparticles (BDM) with a spherical shape, retention of 64,48% of βC, and LGG survivor above 90%. The BDM showed high storage stability for 90 days at room temperature and, at the dose of 2000 mg/kg of BDM did not cause any acute toxicity in Wistar rats. In addition, at the same dose, presented significant anti-inflammatory activity in carrageenan-induced paw edema and pleurisy. Thus, the produced BDM could be an innovative ingredient with functional properties and also an efficient encapsulation strategy for βC.

利用新型双相包囊技术共同包囊益生菌鼠李糖GG和β-胡萝卜素:稳定性和体内抗炎特性
在这项研究中,β-胡萝卜素(βC)被封装在脂质体中,并与益生菌鼠李糖乳杆菌(LGG)一起喷雾干燥,从而获得了具有两种功能成分的双相结构。最初,载入βC的脂质体形成了球形形态的大型多囊泡,平均粒径为1191 nm,夹带效率为81.33%。然后,将 βC-LMVV 与 LGG 的混合物喷雾干燥,得到球形的双相干燥微粒(BDM),βC 的保留率为 64.48%,LGG 的存活率超过 90%。BDM 在室温下 90 天的储存稳定性很高,在剂量为 2000 毫克/千克的情况下,BDM 不会对 Wistar 大鼠造成任何急性毒性。此外,在相同剂量下,BDM 对角叉菜胶引起的爪水肿和胸膜炎具有显著的抗炎活性。因此,生产出的 BDM 可以成为一种具有功能特性的创新成分,同时也是一种有效的 βC 封装策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信