Genomic analysis identifies five pathogenic bacterial species in Argentinian wheat

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences
María Verónica Fumero, Sol Belén Garis, Enrique Alberione, Edgardo Jofré, Leonardo Sebastián Vanzetti
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Abstract

Bacterial diseases significantly impact wheat production worldwide, leading to yield losses ranging from 10 to 40% under diverse climatic conditions. This study aimed to investigate the genomic diversity of bacterial species associated with foliar lesions on wheat in Argentina. Forty foliar lesions from ten plants were collected and a total of forty strains were isolated. Five pathogenic bacterial species were isolated from the lesions, and their genomes were sequenced and assembled. Species identities were confirmed through average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons with type species. The isolated species were identified as follows: Pantoea ananatis ARGTR 1–1 (98% ANI, 82% dDDH), Curtobacterium flaccumfaciens pv. flaccumfaciens ARGTR 5–2 (98% ANI, 90% dDDH), Xanthomonas translucens pv. undulosa ARGTR 7–1 (100% ANI, 99% dDDH), Clavibacter tessellarius ARGTR 8–1 (98% ANI, 97% dDDH), and Pseudomonas syringae pv. atrofaciens ARGTR 9–1 (99% ANI, 91% dDDH). It was notable that Xanthomonas translucens pv. undulosa ARGTR 7–1 and Clavibacter tessellarius ARGTR 8–1 were isolated from the same plant. This study provides a comprehensive phylogenetic analysis and whole-genome data of these bacterial pathogens, establishing a basis for omics-based approaches to explore genetic diversity and virulence mechanisms. These findings would contribute to the development of strategies aimed at improving wheat health and productivity in the presence of bacterial diseases.

Abstract Image

基因组分析确定阿根廷小麦中的五种病原细菌
细菌性病害严重影响着全球小麦的生产,在不同的气候条件下会导致 10% 到 40% 的产量损失。本研究旨在调查阿根廷小麦叶片病害相关细菌物种的基因组多样性。研究人员收集了十株小麦的四十个叶片病斑,共分离出四十株细菌。从病斑中分离出五种致病细菌,并对它们的基因组进行了测序和组装。通过平均核苷酸同一性(ANI)和数字 DNA-DNA 杂交(dDDH)与类型菌种进行比较,确认了菌种的身份。分离出的物种鉴定如下Pantoea ananatis ARGTR 1-1 (98% ANI, 82% dDDH),Curtobacterium flaccumfaciens pv. flaccumfaciens ARGTR 5-2 (98% ANI, 90% dDDH),Xanthomonas translucens pv. undulosa ARGTR 7-1 (98% ANI, 90% dDDH)。undulosa ARGTR 7-1(100% ANI,99% dDDH)、Clavibacter tessellarius ARGTR 8-1(98% ANI,97% dDDH)和 Pseudomonas syringae pv. atrofaciens ARGTR 9-1(99% ANI,91% dDDH)。值得注意的是,Xanthomonas translucens pv. undulosa ARGTR 7-1 和 Clavibacter tessellarius ARGTR 8-1 从同一种植物中分离出来。这项研究为这些细菌病原体提供了全面的系统发育分析和全基因组数据,为基于omics的方法探索遗传多样性和毒力机制奠定了基础。这些发现将有助于制定旨在改善小麦健康和提高小麦产量的策略。
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来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
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