María Verónica Fumero, Sol Belén Garis, Enrique Alberione, Edgardo Jofré, Leonardo Sebastián Vanzetti
{"title":"Genomic analysis identifies five pathogenic bacterial species in Argentinian wheat","authors":"María Verónica Fumero, Sol Belén Garis, Enrique Alberione, Edgardo Jofré, Leonardo Sebastián Vanzetti","doi":"10.1007/s40858-024-00680-2","DOIUrl":null,"url":null,"abstract":"<p>Bacterial diseases significantly impact wheat production worldwide, leading to yield losses ranging from 10 to 40% under diverse climatic conditions. This study aimed to investigate the genomic diversity of bacterial species associated with foliar lesions on wheat in Argentina. Forty foliar lesions from ten plants were collected and a total of forty strains were isolated. Five pathogenic bacterial species were isolated from the lesions, and their genomes were sequenced and assembled. Species identities were confirmed through average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons with type species. The isolated species were identified as follows: <i>Pantoea ananatis</i> ARGTR 1–1 (98% ANI, 82% dDDH), <i>Curtobacterium flaccumfaciens</i> pv. <i>flaccumfaciens</i> ARGTR 5–2 (98% ANI, 90% dDDH), <i>Xanthomonas translucens</i> pv. <i>undulosa</i> ARGTR 7–1 (100% ANI, 99% dDDH), <i>Clavibacter tessellarius</i> ARGTR 8–1 (98% ANI, 97% dDDH), and <i>Pseudomonas syringae</i> pv. <i>atrofaciens</i> ARGTR 9–1 (99% ANI, 91% dDDH). It was notable that <i>Xanthomonas translucens</i> pv. <i>undulosa</i> ARGTR 7–1 and <i>Clavibacter tessellarius</i> ARGTR 8–1 were isolated from the same plant. This study provides a comprehensive phylogenetic analysis and whole-genome data of these bacterial pathogens, establishing a basis for omics-based approaches to explore genetic diversity and virulence mechanisms. These findings would contribute to the development of strategies aimed at improving wheat health and productivity in the presence of bacterial diseases.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00680-2","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Bacterial diseases significantly impact wheat production worldwide, leading to yield losses ranging from 10 to 40% under diverse climatic conditions. This study aimed to investigate the genomic diversity of bacterial species associated with foliar lesions on wheat in Argentina. Forty foliar lesions from ten plants were collected and a total of forty strains were isolated. Five pathogenic bacterial species were isolated from the lesions, and their genomes were sequenced and assembled. Species identities were confirmed through average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) comparisons with type species. The isolated species were identified as follows: Pantoea ananatis ARGTR 1–1 (98% ANI, 82% dDDH), Curtobacterium flaccumfaciens pv. flaccumfaciens ARGTR 5–2 (98% ANI, 90% dDDH), Xanthomonas translucens pv. undulosa ARGTR 7–1 (100% ANI, 99% dDDH), Clavibacter tessellarius ARGTR 8–1 (98% ANI, 97% dDDH), and Pseudomonas syringae pv. atrofaciens ARGTR 9–1 (99% ANI, 91% dDDH). It was notable that Xanthomonas translucens pv. undulosa ARGTR 7–1 and Clavibacter tessellarius ARGTR 8–1 were isolated from the same plant. This study provides a comprehensive phylogenetic analysis and whole-genome data of these bacterial pathogens, establishing a basis for omics-based approaches to explore genetic diversity and virulence mechanisms. These findings would contribute to the development of strategies aimed at improving wheat health and productivity in the presence of bacterial diseases.
期刊介绍:
Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.
Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection.
The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines.
Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.