Multiomics analysis reveals the potential mechanism of high-fat diet in dextran sulfate sodium-induced colitis mice model

IF 3.5 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Science & Nutrition Pub Date : 2024-08-30 DOI:10.1002/fsn3.4426

Yuyang Zhao, Zhimin Chen, Ruiyi Dong, Yufan Liu, Yixin Zhang, Yan Guo, Meiyi Yu, Xiang Li, Jiangbin Wang

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Abstract

A high-fat diet (HFD) is recognized as an important contributor to inflammatory bowel disease (IBD). However, the precise underlying mechanism of HFD on IBD remains elusive. This study aimed to investigate the potential mechanism by which HFD affects IBD using 16S rRNA-sequencing and RNA-seq technology. Results indicated that HFD-treated mice exhibited notable alternations in the structure and composition of the gut microbiota, with some of these alternations being associated with the pathogenesis of IBD. Analysis of the colon transcriptome revealed 11 hub genes and 7 hub pathways among control, DSS-induced colitis, and HFD + DSS-treated groups. Further analysis explores the relationship between the hub pathways and genes, as well as the hub genes and gut microbiota. Overall, the findings indicate that the impact of HFD on DSS-induced colitis may be linked to intestinal dysbiosis and specific genes such as Abca8b, Ace2, Apoa1, Apoa4, Apoc3, Aspa, Dpp4, Maob, Slc34a2, Slc7a9, and Trpm6. These results provide valuable insights for determining potential therapeutic targets for addressing HFD-induced IBD.

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多组学分析揭示高脂饮食在葡聚糖硫酸钠诱导的结肠炎小鼠模型中的潜在作用机制

高脂饮食（HFD）被认为是导致炎症性肠病（IBD）的重要因素。然而，高脂饮食对 IBD 影响的确切机制仍不明确。本研究旨在利用 16S rRNA 序列和 RNA-seq 技术研究 HFD 影响 IBD 的潜在机制。结果表明，经 HFD 处理的小鼠肠道微生物群的结构和组成发生了显著变化，其中一些变化与 IBD 的发病机制有关。对结肠转录组的分析发现，在对照组、DSS诱导的结肠炎组和HFD + DSS处理组中，有11个枢纽基因和7条枢纽通路。进一步的分析探讨了枢纽通路和基因之间的关系，以及枢纽基因和肠道微生物群之间的关系。总之，研究结果表明，HFD 对 DSS 诱导的结肠炎的影响可能与肠道菌群失调和特定基因（如 Abca8b、Ace2、Apoa1、Apoa4、Apoc3、Aspa、Dpp4、Maob、Slc34a2、Slc7a9 和 Trpm6）有关。这些结果为确定潜在的治疗靶点以解决高频分解诱导的 IBD 提供了宝贵的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Science & Nutrition Agricultural and Biological Sciences-Food Science

CiteScore

7.40

自引率

5.10%

发文量

434

审稿时长

24 weeks

期刊介绍： Food Science & Nutrition is the peer-reviewed journal for rapid dissemination of research in all areas of food science and nutrition. The Journal will consider submissions of quality papers describing the results of fundamental and applied research related to all aspects of human food and nutrition, as well as interdisciplinary research that spans these two fields.