Curved multiplanar reformatting allows the accurate histological delineation of hippocampal subfields

IF 2.4 3区医学 Q3 NEUROSCIENCES

Hippocampus Pub Date : 2024-09-11 DOI:10.1002/hipo.23637

Devon James Hupka, Andrew Abey, Ehsan Misaghi, Justine Gargula, Trevor Adam Steve

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Abstract

Background

Hippocampal subfields perform specific roles in normal cognitive functioning and have distinct vulnerabilities in neurological disorders. However, measurement of subfields with MRI is technically difficult in the head and tail of the hippocampus. Recent studies have utilized curved multiplanar reconstruction (CMPR) to improve subfield visualization in the head and tail, but this method has not yet been applied to histological data.

Methods

We utilized BigBrain data, an open-source database of serially sectioned histological data for our analyses. The left hippocampus was segmented according to histological criteria by two raters in order to evaluate intra- and inter-rater reliability of histology-based segmentation throughout the long axis. Segmentation according to our previous protocol for the hippocampal body was then compared to these histological measurements to evaluate for histological validity. Agreement between segmentations was evaluated using Dice similarity coefficients (DSCs).

Results

Intra-rater reliability (DSCs) of histological segmentation was excellent for all subfields: CA1 (0.8599), CA2 (0.7586), CA3/CA4/DG (0.8907), SLM (0.9123), subiculum (0.8149). Similarly, inter-rater reliability analysis demonstrated excellent agreement (DSCs) for all subfield locations: CA1 (0.8203), CA2 (0.7253), CA3/CA4/DG (0.8439), SLM (0.8700), subiculum (0.7794). Finally, histological accuracy (DSCs) for our previous protocol was excellent for all subfields: CA1 (0.8821), CA2 (0.8810), CA3/CA4/DG (0.9802), SLM (0.9879), subiculum (0.8774). When subfields in the hippocampus head, body, and tail were analyzed independently, DSCs also showed excellent agreement.

Conclusions

CMPR allows reliable subfield segmentation based on histological criteria throughout the hippocampal head, body, and tail. Our previous protocol for the hippocampal body can be applied to provide histologically valid subfield measurements throughout the entire hippocampal long axis.

Abstract Image

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曲面多平面重制可准确划分海马亚区的组织学轮廓

背景海马亚区在正常认知功能中发挥着特定的作用，在神经系统疾病中具有明显的脆弱性。然而，用核磁共振成像测量海马头部和尾部的亚场在技术上有困难。最近的研究利用曲线多平面重建（CMPR）来改善头部和尾部的亚场可视化，但这种方法尚未应用于组织学数据。由两名评分员根据组织学标准对左侧海马进行分割，以评估基于组织学的整个长轴分割在评分员内部和评分员之间的可靠性。然后将根据我们之前的方案对海马体进行的分割与这些组织学测量结果进行比较，以评估组织学的有效性。结果在所有亚区中，组织学分割的校内可靠性（DSCs）都非常高，CA1（0.8599）、CA2（0.8599）、CA3（0.8599）、CA4（0.8599）、CA5（0.8599）、CA6（0.8599）：CA1 (0.8599)、CA2 (0.7586)、CA3/CA4/DG (0.8907)、SLM (0.9123)、subiculum (0.8149)。同样，评分者之间的可靠性分析表明，所有子场位置的一致性（DSCs）都非常好：CA1（0.8203）、CA2（0.7253）、CA3/CA4/DG（0.8439）、SLM（0.8700）、子网（0.7794）。最后，我们先前方案的组织学准确性（DSCs）在所有子场中都非常出色：CA1（0.8821）、CA2（0.8810）、CA3/CA4/DG（0.9802）、SLM（0.9879）、网下（0.8774）。结论CMPR 可以根据组织学标准对整个海马头、体和尾进行可靠的亚区分割。我们之前针对海马体的方案可用于对整个海马长轴进行组织学上有效的子野测量。

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来源期刊

Hippocampus 医学-神经科学

CiteScore

5.80

自引率

5.70%

发文量

审稿时长

3-8 weeks

期刊介绍： Hippocampus provides a forum for the exchange of current information between investigators interested in the neurobiology of the hippocampal formation and related structures. While the relationships of submitted papers to the hippocampal formation will be evaluated liberally, the substance of appropriate papers should deal with the hippocampal formation per se or with the interaction between the hippocampal formation and other brain regions. The scope of Hippocampus is wide: single and multidisciplinary experimental studies from all fields of basic science, theoretical papers, papers dealing with hippocampal preparations as models for understanding the central nervous system, and clinical studies will be considered for publication. The Editor especially encourages the submission of papers that contribute to a functional understanding of the hippocampal formation.