QuEChERS-HPLC-MS for the determination of coronatine residues in paddy field environment and five common fruits and vegetables

Abstract

The residue of coronatine in the paddy field environment, as well as five common fruits and vegetables, was analyzed using the QuEChERS pre-treatment technique combined with HPLC-MS in this experiment. Acetonitrile was used as the extractant, while C and multi-walled carbon nanotubes served as adsorbents for sample extraction and purification. Subsequently, the samples were filtered through a 0.2 μm organic filter membrane before detection on the machine. The limits of detection (LOD) and quantification (LOQ) for coronatine in the paddy field environment and five common fruits and vegetables were determined to be 0.0005–0.0035 mg/kg and 0.0018–0.0118 mg/kg, respectively, exhibiting good linear correlation coefficients (R≥0.9900) at spiked levels ranging from 0.01 to 1 mg/kg. The average intra-day recoveries ranged from 75.19 % to 107.9 %, with relative standard deviations (RSDs) of 1.24 %-12.66 % at spiked levels of 0.05–0.5 mg/kg.