Exploration of the Therapeutic Potential of Salvianolic Acid B Against senile Cataracts Based on Network Pharmacology and Experimental Validation

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Natural Product Communications Pub Date : 2024-08-30 DOI:10.1177/1934578x241272458

Yongxiao Dong, Jin Zhao, Xueli Zheng, Tao Xue, Wenting Ma, Panpan Cao, Ling Wang, Xiaoyong Yuan

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引用次数: 0

Abstract

BackgroundThe aim of this study was to explore the preventive and therapeutic potential of salvianolic acid B in inhibiting senile cataracts through network pharmacology and experimental validation.MethodsDisease-related genes were obtained from the DisGeNET and GeneCards databases. Drug targets were identified from the Swiss Target Prediction and PharmMapper databases, shared genes were identified via the Venny website, links between genes were identified via a protein–protein interaction (PPI) network, and GO and KEGG analyses were subsequently performed via R software. The key genes were identified via Cytoscape software, and their binding with Sal-B was demonstrated by molecular docking. Then, the results were verified by cell experiments. A CCK-8 assay was used to assess the activity of human LECs with or without H2O2 or Sal-B treatment, and the cell apoptosis rate of each group was determined by flow cytometry. The gene expression levels of caspase 3, TNF-α and MMP-9 in human LECs treated with or without H2O2 or Sal-B were determined by qPCR.ResultsA total of 705 and 152 cataract-related genes and salvianolic acid B-related genes, respectively, were identified, with 37 shared genes. The PPI results showed that MMP9, IL-2, JUN, TNF-α and caspase 3 were the core genes. GO data analysis revealed that the biological process, cell component and molecular function terms were most enriched in the categories “apoptosis progress”, “cytosol” and “protein binding”. Through KEGG enrichment analysis, we found that the core genes were related to the IL-17 and TNF signalling pathways. Cytoscape results showed that MMP9, TNF-α and caspase 3 were the key genes, and molecular docking showed that the drugs interacted well with the target molecules. The experimental results showed that salvianolic acid B inhibited H2O2-induced decreases in LEC activity and apoptosis and inhibited the gene expression of MMP9, TNF-α and caspase 3.ConclusionNetwork pharmacology and molecular docking results showed that salvianolic acid B has the potential to prevent and treat senile cataracts. The experimental results verified the finding that salvianolic acid B can inhibit the decrease in LEC activity and apoptosis induced by H2O2 and verified the expression of the key molecules MMP9, TNF-α and caspase 3.

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基于网络药理学和实验验证的丹酚酸 B 对老年性白内障的治疗潜力探索

背景本研究旨在通过网络药理学和实验验证，探索丹酚酸 B 在抑制老年性白内障方面的预防和治疗潜力。方法从 DisGeNET 和 GeneCards 数据库中获取疾病相关基因。从 Swiss Target Prediction 和 PharmMapper 数据库中确定药物靶点，通过 Venny 网站确定共享基因，通过蛋白-蛋白相互作用（PPI）网络确定基因之间的联系，随后通过 R 软件进行 GO 和 KEGG 分析。通过 Cytoscape 软件确定了关键基因，并通过分子对接证明了它们与 Sal-B 的结合。然后，通过细胞实验对结果进行了验证。CCK-8检测法用于评估经H2O2或Sal-B处理或未经H2O2或Sal-B处理的人LECs的活性，流式细胞仪测定各组细胞的凋亡率。采用 qPCR 法测定 H2O2 或 Sal-B 处理或未处理的人 LECs 中 Caspase 3、TNF-α 和 MMP-9 的基因表达水平。PPI结果显示，MMP9、IL-2、JUN、TNF-α和caspase 3是核心基因。GO数据分析显示，生物过程、细胞组分和分子功能术语在 "凋亡进展"、"细胞膜 "和 "蛋白质结合 "类别中富集最多。通过 KEGG 富集分析，我们发现核心基因与 IL-17 和 TNF 信号通路有关。Cytoscape结果显示，MMP9、TNF-α和caspase 3是关键基因，分子对接显示药物与靶分子有良好的相互作用。实验结果表明，丹酚酸 B 可抑制 H2O2 诱导的 LEC 活性下降和细胞凋亡，抑制 MMP9、TNF-α 和 caspase 3 的基因表达。实验结果验证了丹酚酸 B 能抑制 H2O2 诱导的 LEC 活性下降和细胞凋亡，并验证了关键分子 MMP9、TNF-α 和 caspase 3 的表达。

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来源期刊

Natural Product Communications 工程技术-食品科技

CiteScore

3.10

自引率

11.10%

发文量

254

审稿时长

2.7 months

期刊介绍： Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products.