Antioxidant, Anti-Inflammatory, and Anti-Bacterial Activities of Artemisia vulgaris L. Essential Oil in Vietnam

IF 1.5 4区医学 Q4 CHEMISTRY, MEDICINAL

Natural Product Communications Pub Date : 2024-08-30 DOI:10.1177/1934578x241275782

Pham Thi Nhat Trinh, Le Xuan Tien, Tong Thanh Danh, Dang Thi Le Hang, Nguyen Van Hoa, To Thi Bao Yen, Le Tien Dung

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引用次数: 0

Abstract

BackgroundArtemisia vulgaris L. (AV) is a beneficial herb with therapeutic properties. This work aims to evaluate the compositions and bioactivity of essential oil from AV grown in Tiengiang, Vietnam.MethodsThe essential oils (AVEO) were extracted by hydrodistillation (HD), and the headspace volatiles (HS) were collected using the static headspace technique. Gas chromatography/mass spectrometry (GC/MS) was used to examine the compositions of oils in detail. The agar well-diffusion technique was used to conduct the antibacterial test. Multi-concentration dilution method was used for minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determination. The cell viability was determined by the MTT method. Nitric oxide and TNF-α in RAW 264.7 were analyzed by Griess reagent or ELISA as described by the manufacturer. In addition, DPPH, ABTS, and reducing power assay were used to determine the antioxidant activity.ResultsThe HD method yielded 1,8-cineole, α-pinene, β -caryophyllene, borneol, camphor, and δ-elemene were the most abundant components. In contrast, the HS approach produced the primary constituents being camphor, 2-methylbutanal, 1,8-cineole, and camphene. The HD showed antibacterial activities against E.coli, S.typhimurium, S .aureus, P.aeruginosa, and S.mutans, and inhibited the production of NO, TNF-α in lipopolysaccharide-induced RAW264.7. Furthermore, the HD indicated moderate antioxidant activity. A hierarchical cluster analysis of essential oils from 22 regions shows that mugwort oils in Tiengiang, Vietnam, have a high level of δ-elemene (more than 5%), which is not common in oils from other species. The study's findings enhance our comprehension of the chemical components and chemical variety of mugwort oil across different sites and to help in the identification of the predominant species suitable for extracting essential oils for different purposes within the Artemisia vulgaris L. species.ConclusionThese findings collectively suggest that essential oils derived from A. vulgaris have a range of potential therapeutic uses.

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越南青蒿精油的抗氧化、抗炎和抗菌活性

背景青蒿（Artemisia vulgaris L.，AV）是一种具有治疗功效的有益草药。本研究旨在评估生长在越南天江（Tiengiang）的青蒿精油的成分和生物活性。方法采用水蒸馏法（HD）提取青蒿精油（AVEO），并利用静态顶空技术收集顶空挥发物（HS）。气相色谱/质谱法（GC/MS）用于详细检测精油的成分。采用琼脂井扩散技术进行抗菌测试。采用多浓度稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。细胞活力用 MTT 法测定。RAW 264.7 中的一氧化氮和 TNF-α 用 Griess 试剂或 ELISA 进行分析，具体方法由生产商提供。结果 HD 方法产生的 1,8-蒎烯、α-蒎烯、β-石竹烯、龙脑、樟脑和 δ-榄香烯是最丰富的成分。相比之下，HS 方法产生的主要成分是樟脑、2-甲基丁醛、1,8-蒎烯和莰烯。HD 对大肠杆菌、伤寒杆菌、金黄色葡萄球菌、铜绿假单胞菌和变异单胞菌具有抗菌活性，并能抑制脂多糖诱导的 RAW264.7 中 NO 和 TNF-α 的产生。此外，HD 还具有适度的抗氧化活性。对来自 22 个地区的精油进行的分层聚类分析显示，越南天江的艾草精油中含有大量δ-榄香烯（超过 5%），这在其他物种的精油中并不常见。这项研究结果提高了我们对不同地区艾草精油的化学成分和化学多样性的理解，有助于确定适合提取精油用于不同用途的粗艾蒿的主要品种。

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来源期刊

Natural Product Communications 工程技术-食品科技

CiteScore

3.10

自引率

11.10%

发文量

254

审稿时长

2.7 months

期刊介绍： Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products. Natural Product Communications is a peer reviewed, open access journal studying all aspects of natural products, including isolation, characterization, spectroscopic properties, biological activities, synthesis, structure-activity, biotransformation, biosynthesis, tissue culture and fermentation. It covers the full breadth of chemistry, biochemistry, biotechnology, pharmacology, and chemical ecology of natural products.