Integrated transcriptome and metabolome analyses reveal anthocyanin biosynthesis in red and green mango pericarps under light and shade conditions

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2024-09-10 DOI:10.1016/j.scienta.2024.113617

Jingxian Chen , Wenting Wu , Siwei Gao , Mohammad Shah Jahan , Jincan Xiao , Tianli Guo , Canbin Chen , Baijun Li , Cong Luo , Xinhua He , Fangfang Xie

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引用次数: 0

Abstract

Anthocyanin is a natural plant pigment whose biosynthesis is intricately linked to light regulation. Here, pericarps of red mango cultivars named 'Xinshiji (XSJ)' and 'Hongmang No.6 (HM6)' directly exposed to sunlight were red while they were green under shade conditions, whereas the pericarps of green mango cultivar known as 'Guiqi (GQ)' maintained green within both light and shade conditions. However, the mechanisms underlying these color changes under different light conditions remain largely unexplored. Therefore, we analyzed the contents, components, and biosynthesis of anthocyanins in the above mango pericarps through metabolome and transcriptome analyses. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis releaved a total of 35 anthocyanin compounds were detected in the mango pericarps. Transcriptome analysis uncovered 44 differentially expressed genes among the comparison of 'XSJ', 'HM6', and 'GQ' samples. Twelve and eight candidate genes were identified in the anthocyanin biosynthesis and chlorophyll breakdown pathways, respectively. A co-expression network of the key anthocyanin biosynthetic genes revealed that twelve transcription factors may govern anthocyanin biosynthesis and chlorophyll breakdown processes under light. These candidate genes were further verified by real-time quantitative PCR (RT-qPCR) for providing a regulatory model for the formation of red-and green-pericarps in mangoes under light and shade conditions. These results deepen our understanding of light-induced anthocyanin biosynthesis in fruits.

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转录组和代谢组的综合分析揭示了红芒和绿芒果皮在光照和遮荫条件下的花青素生物合成过程

花青素是一种天然植物色素，其生物合成与光照调节密切相关。在这里，直接暴露在阳光下的红芒果栽培品种 "新世纪（XSJ）"和 "红芒 6 号（HM6）"的果皮呈红色，而在遮光条件下则呈绿色；而绿芒果栽培品种 "桂七（GQ）"的果皮在光照和遮光条件下均保持绿色。然而，这些在不同光照条件下颜色变化的机理在很大程度上仍未得到研究。因此，我们通过代谢组和转录组分析，对上述芒果果皮中花青素的含量、成分和生物合成进行了分析。通过高效液相色谱-串联质谱（HPLC-MS/MS）分析，我们在芒果果皮中检测到了 35 种花青素化合物。转录组分析发现，在 "XSJ"、"HM6 "和 "GQ "样品的比较中，有 44 个基因表达不同。在花青素生物合成和叶绿素分解途径中分别发现了 12 个和 8 个候选基因。花青素生物合成关键基因的共表达网络显示，有 12 个转录因子可能控制着光照下的花青素生物合成和叶绿素分解过程。通过实时定量 PCR（RT-qPCR）进一步验证了这些候选基因，为芒果在光照和遮荫条件下形成红色和绿色果皮提供了一个调控模型。这些结果加深了我们对光照诱导果实花青素生物合成的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.