Recombinant protein LPxT-GYLEQ attenuates cognitive impairment by ameliorating oxidative stress in D-galactose-induced aging mice model

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Yiping Yang , Shuyi Lu , Yu Liang , Xubin Tu , Xiaoqun Zeng , Li Wang , Daodong Pan , Tao Zhang , Zhen Wu
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Abstract

Food-derived antioxidant peptides have been shown to have beneficial effects in scavenging excess free radicals. In this study, a novel multifunctional LPxTG-motif protein LPxT-GYLEQ was synthesized, and its molecular mechanism of alleviating cognitive impairment in a D-galactose (D-gal)-induced aging mice model was also investigated. The results confirmed the antioxidant effects of the LPxT-GYLEQ protein, which could scavenge excessive reactive oxygen species (ROS) in aging mice by regulating the c-Jun N-terminal kinase (JNK)/Nuclear factor erythroid 2-related factor 2 (Nrf2)/p38/Nuclear factor-k-gene binding (NF-κB) signal pathway, reduced the accumulation of β-amyloid protein (Aβ), restored the cognitive ability of mice, improved learning and memory behavior, effectively reduced the expression of inflammatory-related factors, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), and increased the expression of anti-inflammatory factor interleukin-10 (IL-10). These may be related to the fact that the protein regulates the abundance of beneficial bacteria such as intestinal Akkermansia Muciniphila (Akk). All results suggest that the synthetic LPxT-GYLEQ protein may improve cognitive impairment and be a promising candidate for an anti-aging agent.

Abstract Image

重组蛋白 LPxT-GYLEQ 通过改善 D-半乳糖诱导的衰老小鼠模型中的氧化应激减轻认知障碍
从食物中提取的抗氧化肽已被证明具有清除过量自由基的有益作用。本研究合成了一种新型多功能LPxTG-motif蛋白LPxT-GYLEQ,并研究了它在D-半乳糖(D-gal)诱导的衰老小鼠模型中缓解认知障碍的分子机制。结果证实了LPxT-GYLEQ蛋白的抗氧化作用,它可以通过调节c-Jun N-末端激酶(JNK)/核因子红细胞2相关因子2(Nrf2)/p38/核因子-基因结合(NF-κB)信号通路,清除衰老小鼠体内过量的活性氧(ROS)、减少了β淀粉样蛋白(Aβ)的积累,恢复了小鼠的认知能力,改善了学习和记忆行为,有效降低了肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)等炎症相关因子的表达,增加了抗炎因子白细胞介素-10(IL-10)的表达。这些可能与该蛋白质调节肠道 Akkermansia Muciniphila(Akk)等有益细菌的数量有关。所有结果都表明,合成的 LPxT-GYLEQ 蛋白可能会改善认知障碍,并有望成为一种抗衰老剂。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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