Structural characterization and prebiotic activity evaluation of novel fucosylated disaccharides prepared from bacterial exopolysaccharides

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
{"title":"Structural characterization and prebiotic activity evaluation of novel fucosylated disaccharides prepared from bacterial exopolysaccharides","authors":"","doi":"10.1016/j.fbio.2024.105055","DOIUrl":null,"url":null,"abstract":"<div><p>The deoxyhexose sugar <span>l</span>-fucose and fucosylated oligosaccharides (FCO) are essential for various biological processes in human body and associated gut microbiota. Bacterial exopolysaccharides offer a promising new source for preparing functional oligosaccharides. Here, FCO were obtained through acidolysis of fucose-containing exopolysaccharides with a molecular weight of 5.9 × 10<sup>3</sup> kDa, derived from <em>Clavibacter michiganensis</em> M2. Structural analysis revealed that the main disaccharide components of FCO exhibited a molecular weight of 326 Da and a monosaccharide composition of fucose, galactose, and glucose in a 3:1:2 ratio. Nuclear magnetic resonance indicated that the disaccharide fragments consisted of two backbones of α-<em>Galp</em>-(1 → 2)-α-<em>Fucp</em> and β-<em>Glcp</em>-(1 → 4)-β-<em>Fucp</em>, respectively. <em>In vitro</em> fermentation of FCO with human fecal cultures resulted in an increased abundance of <em>Bifidobacterium</em> and <em>Bacteroides</em>, and a reduction in <em>Escherichia-Shigella</em> and <em>Fusobacterium</em>. This research outlines a novel structural fucosylated oligosaccharide and its preparation method, suggesting its potential application as a promising new prebiotic.</p></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Bioscience","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212429224014858","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The deoxyhexose sugar l-fucose and fucosylated oligosaccharides (FCO) are essential for various biological processes in human body and associated gut microbiota. Bacterial exopolysaccharides offer a promising new source for preparing functional oligosaccharides. Here, FCO were obtained through acidolysis of fucose-containing exopolysaccharides with a molecular weight of 5.9 × 103 kDa, derived from Clavibacter michiganensis M2. Structural analysis revealed that the main disaccharide components of FCO exhibited a molecular weight of 326 Da and a monosaccharide composition of fucose, galactose, and glucose in a 3:1:2 ratio. Nuclear magnetic resonance indicated that the disaccharide fragments consisted of two backbones of α-Galp-(1 → 2)-α-Fucp and β-Glcp-(1 → 4)-β-Fucp, respectively. In vitro fermentation of FCO with human fecal cultures resulted in an increased abundance of Bifidobacterium and Bacteroides, and a reduction in Escherichia-Shigella and Fusobacterium. This research outlines a novel structural fucosylated oligosaccharide and its preparation method, suggesting its potential application as a promising new prebiotic.

Abstract Image

从细菌外多糖制备的新型岩藻糖基化二糖的结构表征和益生活性评估
脱氧己糖 l-岩藻糖和岩藻糖基化低聚糖(FCO)是人体和相关肠道微生物群各种生物过程所必需的。细菌外多糖为制备功能性寡糖提供了一种前景广阔的新来源。本文通过酸解含岩藻糖的外多糖获得了 FCO,其分子量为 5.9 × 103 kDa,来源于 Clavibacter michiganensis M2。结构分析表明,FCO 的主要双糖成分分子量为 326 Da,单糖成分为岩藻糖,半乳糖和葡萄糖,比例为 3:1:2。核磁共振显示,二糖片段分别由 α-Galp-(1 → 2)-α-Fucp 和 β-Glcp-(1 → 4)-β-Fucp 两种骨架组成。用人类粪便培养物对 FCO 进行体外发酵,可增加双歧杆菌和乳杆菌的数量,减少志贺氏菌和镰刀菌的数量。这项研究概述了一种结构新颖的岩藻糖基化寡糖及其制备方法,表明它有可能被用作一种前景广阔的新型益生元。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信