A novel antimicrobial peptide MP-4: Insights into its antimicrobial properties and intestinal regulation on E. coli-infected mice

IF 4.8 1区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Mengmeng Zhang , Quan Shuang , Kunjie Guo , Fengmei Zhang , Yanan Xia
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引用次数: 0

Abstract

The quest for novel, potent antimicrobial agents with low resistance potential poses a significant challenge for the advancement of the food and medical sectors. This study aimed to elucidate the antibacterial potency of antimicrobial peptides derived from koumiss in a murine model. Leveraging an antimicrobial peptide database, six peptides (MP-1 to MP-6) were meticulously predicted and screened for their antibacterial properties. These peptides were subsequently synthesized using chemical solid-phase methods and their antibacterial activities were rigorously validated. Remarkably, among the six peptides, MP-4 demonstrated a profound antibacterial effect against E. coli, achieving rapid bacterial eradication within 240 min. Flow cytometry analysis further corroborated its significant bactericidal activity. In vivo experiments conducted on mice infected with E. coli revealed that oral administration of MP-4 significantly ameliorated symptoms such as lethargy, anorexia, and weight loss. Additionally, it effectively reduced the colonic E. coli burden, attenuated inflammatory responses, and favorably modulated the intestinal microbiota composition. This study not only validates the robust antibacterial activity of the koumiss-derived antimicrobial peptide MP-4, but also underscores its potential therapeutic application in mitigating E. coli infections and promoting intestinal health.

新型抗菌肽 MP-4:其抗菌特性及其对大肠杆菌感染小鼠肠道调节的启示
寻找抗药性低的新型强效抗菌剂对食品和医疗行业的发展构成了重大挑战。本研究的目的是在小鼠模型中阐明提取自 koumiss 的抗菌肽的抗菌效力。利用抗菌肽数据库,对六种肽(MP-1 至 MP-6)的抗菌特性进行了细致的预测和筛选。随后采用化学固相方法合成了这些多肽,并对它们的抗菌活性进行了严格验证。值得注意的是,在这六种多肽中,MP-4 对大肠杆菌具有显著的抗菌效果,能在 240 分钟内迅速消灭细菌。流式细胞仪分析进一步证实了其显著的杀菌活性。在感染了大肠杆菌的小鼠身上进行的体内实验显示,口服 MP-4 能明显改善小鼠嗜睡、厌食和体重减轻等症状。此外,MP-4 还能有效减少结肠大肠杆菌的负担,减轻炎症反应,并对肠道微生物群的组成产生有利的调节作用。这项研究不仅验证了源自库米斯的抗菌肽 MP-4 的强大抗菌活性,还强调了它在减轻大肠杆菌感染和促进肠道健康方面的潜在治疗应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Food Bioscience
Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
6.40
自引率
5.80%
发文量
671
审稿时长
27 days
期刊介绍: Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.
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