Encapsulation of Candida antarctica lipase B in metal-organic framework under ultrasound and using it to one-pot synthesis of 1,3,4,5-tetrasubstituted pyrazoles.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2024-12-01 Epub Date: 2024-09-06 DOI:10.1007/s00449-024-03083-4

Zeynab Rangraz, Ali Jafari, Mansour Shahedi, Mostafa M Amini, Zohreh Habibi

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Abstract

Encapsulating the enzyme in metal-organic frameworks (MOFs) is a convenient method to prepare MOF-enzyme biocomposite. In this study, Candida antarctica lipase B (CAL-B) was chosen to immobilize in Cu-BTC MOF under ultrasound irradiation. CAL-B was immobilized in Cu-BTC under ultrasound at 21 kHz and 11.4 W/cm² and incubation. 98% of CAL-B was immobilized in Cu-BTC with 99 U/mg activity (threefold more active than the free CAL-B). The prepared biocomposite was characterized using FT-IR, XRD, TGA, SEM, EDX, and BET. The thermal and solvent stability of CAL-B@Cu-BTC was investigated. It was found that at a temperature of 55 ℃, CAL-B@Cu-BTC maintains its activity even after 2 h of incubation. Furthermore, in the presence of 20% and 50% concentrations of MeCN, THF, and DMF, CAL-B@Cu-BTC was found to have an activity of over 80%. A prepared biocatalyst was used to synthesize 1,3,4,5-tetrasubstituted pyrazole derivatives (50-75%) in a one-pot vessel, by adding phenyl hydrazine hydrochlorides, benzaldehydes, and dimethyl acetylenedicarboxylate.

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在超声波作用下在金属有机框架中封装白色念珠菌脂肪酶 B，并利用它一锅合成 1,3,4,5 四代吡唑。

将酶封装在金属有机框架（MOF）中是制备 MOF-酶生物复合材料的一种简便方法。本研究选择了白色念珠菌脂肪酶 B（CAL-B）在超声辐照下固定在 Cu-BTC MOF 中。在 21 kHz 和 11.4 W/cm2 的超声波和培养条件下，CAL-B 被固定在 Cu-BTC MOF 中。98% 的 CAL-B 被固定在 Cu-BTC 中，其活性为 99 U/mg （比游离的 CAL-B 活性高三倍）。利用傅立叶变换红外光谱（FT-IR）、X射线衍射（XRD）、热重分析（TGA）、扫描电镜（SEM）、电离辐射分析（EDX）和BET对制备的生物复合材料进行了表征。研究了 CAL-B@Cu-BTC 的热稳定性和溶剂稳定性。结果发现，在 55 ℃ 的温度下，CAL-B@Cu-BTC 即使在培养 2 小时后仍能保持其活性。此外，在 20% 和 50% 浓度的 MeCN、THF 和 DMF 存在下，CAL-B@Cu-BTC 的活性超过 80%。利用制备的生物催化剂，通过加入苯肼盐酸盐、苯甲醛和乙酰二甲酸二甲酯，在一锅容器中合成了 1,3,4,5-四取代的吡唑衍生物（50-75%）。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.