Carbapenemase and extended-spectrum beta-lactamase-producing bacteria in waters originating from a single landfill in Slovenia.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae070

Valerija Tkalec, Polona Lindic, Tatjana Jursa, Hermina Ivanusa Sket, Leon Maric, Mojca Cimerman, Maja Rupnik, Andrej Golle

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Abstract

Groundwater, rainwater, and leachate associated with a single landfill were analysed to detect extended-spectrum beta-lactamase (ESBL)-producing and carbapenemase (CP)-producing bacteria. After cultivation on three commercial selective-differential media, 240 bacterial isolates were obtained and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Isolates from clinically relevant species were further genotyped by enterobacterial repetitive intergenic consensus polymerase chain reaction, and tested for antibiotic susceptibility and presence of CPs and ESBL enzymes. Two ESBL-producing isolates and two isolates producing CPs were detected in rainwater, groundwater, and leachate: Klebsiella oxytoca complex with the gene for the ESBL enzyme CTX-M-1 and the gene for the CP OXA-48, Serratia fonticola with the gene for the ESBL enzyme FONA-2, and Pseudomonas aeruginosa with the gene coding Verona integron-encoded Metallo-beta-lactamases (VIM) metallo-beta-lactamase. Our study indicates that bacteria with ESBL and CP genes can be present in landfill-associated waters.

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斯洛文尼亚单一垃圾填埋场水域中产生碳青霉烯酶和广谱β-内酰胺酶的细菌。

对与一个垃圾填埋场相关的地下水、雨水和沥滤液进行了分析，以检测产生广谱β-内酰胺酶（ESBL）和碳青霉烯酶（CP）的细菌。在三种商业选择性差异培养基上培养后，获得了 240 个细菌分离物，并通过 MALDI-TOF MS 进行了鉴定。通过肠杆菌重复基因间共识（ERIC）聚合酶链式反应（PCR）对临床相关菌种的分离物进行了进一步基因分型，并检测了抗生素敏感性以及碳青霉烯酶和 ESBL 酶的存在。在雨水、地下水和沥滤液中检测到两个产生 ESBL 的分离物和两个产生碳青霉烯酶的分离物：它们分别是：带有 ESBL 酶 CTX-M-1 基因和碳青霉烯酶 OXA-48 基因的复合克雷伯菌、带有 ESBL 酶 FONA-2 基因的丰胸沙雷氏菌和带有 VIM 金属-β内酰胺酶编码基因的铜绿假单胞菌。我们的研究表明，垃圾填埋场相关水体中可能存在带有 ESBL 和 CP 基因的细菌。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.