Therapeutic Effects of Nigella sativa Oil and Whole Seeds on STZ-Induced Diabetic Rats: A Biochemical and Immunohistochemical Study.

2区 生物学 Q1 Biochemistry, Genetics and Molecular Biology
Oxidative Medicine and Cellular Longevity Pub Date : 2024-08-10 eCollection Date: 2024-01-01 DOI:10.1155/2024/5594090
Naif AlSuhaymi
{"title":"Therapeutic Effects of <i>Nigella sativa</i> Oil and Whole Seeds on STZ-Induced Diabetic Rats: A Biochemical and Immunohistochemical Study.","authors":"Naif AlSuhaymi","doi":"10.1155/2024/5594090","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Type II diabetes mellitus (DM) is an increasing health problem that has negative impacts on patients and healthcare systems, worldwide. The development of new therapies with better efficacy, fewer side effects, and lower prices are urgently needed to treat this disease.</p><p><strong>Aim: </strong>To evaluate and compare the therapeutic effects of <i>Nigella sativa</i> (<i>N. sativa</i>) seed and oil on the biochemical parameters and regeneration of pancreatic islets (or islets of Langerhans) of streptozotocin (STZ)-induced diabetic rats.</p><p><strong>Materials and methods: </strong>The diabetic rat model was prepared by administering a single dose of STZ (35 mg/kg body weight). The whole seed or the oil of <i>N. sativa</i> was administered to the diabetic and control groups for a period of 28 days, but not to the negative and STZ controls. Serum blood glucose, liver enzymes, lipid profile, and renal function tests (uric acid, albumin, total protein, urea, and creatinine) were measured in all groups. After the rats were euthanized, their pancreases were extracted, and then sectioned and fixed on slides in preparation before staining with H&E stain and immunohistochemical study.</p><p><strong>Results: </strong>Treatment of STZ-diabetic rats with <i>N. sativa</i> seeds or oil significantly improved their serum glucose levels, lipid profiles, and liver and renal functions as well as preserved the integrity of pancreatic <i>β</i> cells.</p><p><strong>Conclusion: </strong><i>N. sativa</i> seeds and oil demonstrate significant therapeutic improvement effects on DM and its related complications including effective protection of islets of Langerhans. The therapeutic benefits of <i>N. sativa</i> seeds and oil on DM and its related complications are comparable.</p>","PeriodicalId":19657,"journal":{"name":"Oxidative Medicine and Cellular Longevity","volume":"2024 ","pages":"5594090"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11330337/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oxidative Medicine and Cellular Longevity","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1155/2024/5594090","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Type II diabetes mellitus (DM) is an increasing health problem that has negative impacts on patients and healthcare systems, worldwide. The development of new therapies with better efficacy, fewer side effects, and lower prices are urgently needed to treat this disease.

Aim: To evaluate and compare the therapeutic effects of Nigella sativa (N. sativa) seed and oil on the biochemical parameters and regeneration of pancreatic islets (or islets of Langerhans) of streptozotocin (STZ)-induced diabetic rats.

Materials and methods: The diabetic rat model was prepared by administering a single dose of STZ (35 mg/kg body weight). The whole seed or the oil of N. sativa was administered to the diabetic and control groups for a period of 28 days, but not to the negative and STZ controls. Serum blood glucose, liver enzymes, lipid profile, and renal function tests (uric acid, albumin, total protein, urea, and creatinine) were measured in all groups. After the rats were euthanized, their pancreases were extracted, and then sectioned and fixed on slides in preparation before staining with H&E stain and immunohistochemical study.

Results: Treatment of STZ-diabetic rats with N. sativa seeds or oil significantly improved their serum glucose levels, lipid profiles, and liver and renal functions as well as preserved the integrity of pancreatic β cells.

Conclusion: N. sativa seeds and oil demonstrate significant therapeutic improvement effects on DM and its related complications including effective protection of islets of Langerhans. The therapeutic benefits of N. sativa seeds and oil on DM and its related complications are comparable.

黑麦草油和全籽对 STZ 诱导的糖尿病大鼠的治疗作用:生化和免疫组化研究。
背景:II 型糖尿病(DM)是一个日益严重的健康问题,对全世界的患者和医疗系统都造成了负面影响。目的:评估并比较黑麦草(N. sativa)种子和油对链脲佐菌素(STZ)诱导的糖尿病大鼠的生化指标和胰岛(或朗格汉斯胰岛)再生的治疗作用:给糖尿病大鼠注射单剂量 STZ(35 毫克/千克体重),制备糖尿病大鼠模型。糖尿病组和对照组连续 28 天服用 N. sativa 的全籽或油,而阴性对照组和 STZ 对照组不服用 N. sativa 的全籽或油。对所有组的血糖、肝酶、血脂和肾功能(尿酸、白蛋白、总蛋白、尿素和肌酐)进行了测定。大鼠安乐死后,提取胰腺,切片固定在载玻片上,然后进行 H&E 染色和免疫组化研究:结果:用藜芦籽或藜芦油治疗 STZ 糖尿病大鼠,能明显改善其血糖水平、血脂状况、肝肾功能,并能保护胰腺 β 细胞的完整性:结论:荠菜籽和油对糖尿病及其相关并发症有明显的治疗改善作用,包括有效保护朗格汉斯胰岛。荠菜籽和油对 DM 及其相关并发症的治疗效果相当。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
13.20
自引率
0.00%
发文量
1274
审稿时长
3-8 weeks
期刊介绍: Oxidative Medicine and Cellular Longevity is a unique peer-reviewed, Open Access journal that publishes original research and review articles dealing with the cellular and molecular mechanisms of oxidative stress in the nervous system and related organ systems in relation to aging, immune function, vascular biology, metabolism, cellular survival and cellular longevity. Oxidative stress impacts almost all acute and chronic progressive disorders and on a cellular basis is intimately linked to aging, cardiovascular disease, cancer, immune function, metabolism and neurodegeneration. The journal fills a significant void in today’s scientific literature and serves as an international forum for the scientific community worldwide to translate pioneering “bench to bedside” research into clinical strategies.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信