Consolidated bioprocessing of lignocellulosic wastes in Northwest China for D-glucaric acid production by an artificial microbial consortium.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2024-12-01 Epub Date: 2024-08-19 DOI:10.1007/s00449-024-03081-6

Hao Fang, Yuchen Li, Yuqi Song, Liang Yu, Xiangyang Song, Chen Zhao

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Abstract

D-glucaric acid is a platform chemical of great importance and the consolidated bioprocessing (CBP) of lignocellulose by the microbial consortium of Trichoderma reesei C10 and Saccharomyces cerevisiae LGA-1C3S2 features prospects in biomanufacturing it. Here we compared some representative lignocelluloses in Northwest China including corn stover, wheat straw and switchgrass, and the leading pretreatments including steam explosion, subcritical water pretreatment, sodium hydroxide pretreatment, aqueous ammonia pretreatment, lime pretreatment, and diluted sulfuric acid pretreatment. It was found that sodium hydroxide pretreated switchgrass (SHPSG) was the best substrate for D-glucaric acid production, resulting in the highest D-glucaric acid titers, 11.69 ± 0.73 g/L in shake flask and 15.71 ± 0.80 g/L in 10L airlift fermenter, respectively. To the best of our knowledge, this is the highest D-glucaric acid production titer from lignocellulosic biomass. This work offers a paradigm of producing low-cost D-glucaric acid for low-carbon polyethylene 2,5-furandicarboxylate (PEF) and a reference on developing biorefinery in Northwest China.

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利用人工微生物联合体对中国西北地区木质纤维素废物进行综合生物处理，以生产 D-葡萄糖酸。

D-葡糖酸是一种非常重要的平台化学品，由毛霉C10和酿酒酵母LGA-1C3S2组成的微生物联合体对木质纤维素进行综合生物处理（CBP）具有生物制造木质纤维素的前景。在此，我们比较了西北地区具有代表性的木质纤维素（包括玉米秸秆、小麦秸秆和开关草）和主要预处理方法（包括蒸汽爆破、亚临界水预处理、氢氧化钠预处理、水氨预处理、石灰预处理和稀硫酸预处理）。研究发现，经氢氧化钠预处理的开关草（SHPSG）是生产 D-葡糖酸的最佳基质，其 D-葡糖酸滴度最高，在摇瓶中为 11.69 ± 0.73 g/L，在 10L 空运发酵罐中为 15.71 ± 0.80 g/L。据我们所知，这是利用木质纤维素生物质生产 D-葡糖酸的最高滴度。该研究为低碳聚 2,5-呋喃二甲酸乙二醇酯（PEF）生产低成本 D-葡糖酸提供了范例，也为中国西北地区发展生物炼制提供了参考。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.