Antibacterial mode of action of garviecin LG34 against Gram-negative bacterium Salmonella typhimurium.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae066

Yurong Gao, Dapeng Li

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引用次数: 0

Abstract

Garviecin LG34 produced by Lactococcus garvieae LG34 exhibits wide-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria. This work aimed at clarifying the antibacterial mode of action of garviecin LG34 against Gram-negative bacterium Salmonella typhimurium. To determine the concentration for the bacteriocin antimicrobial mode experiments, the minimum inhibitory concentration of garviecin LG34 against S. typhimurium CICC21484 was determined as 0.25 mg/ml. Garviecin LG34 decreased the viable count of S. typhimurium CICC21484 and its antibacterial activity was the dose and time dependant. Garviecin LG34 led to the dissipation of transmembrane potential, the rise in the extracellular conductivity, UV-absorbing material at 260 nm, and LDH level of S. typhimurium CICC21484. Scanning electron micrographs results shown that garviecin LG34 cause dramatic deformation and fragmentation including the flagellum shedding, pores formation in surface, and even completely breakage of S. typhimurium cell. Moreover, garviecin LG34 decreased the intracellular ATP level. The results of this study demonstrated that garviecin LG34 can destroy cell structure, increase membrane permeability of S. typhimurium, thereby might be used as biopreservative for treating food borne and salmonellosis resulting from Gram-negative bacterium S. typhimurium.

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Garviecin LG34 对革兰氏阴性菌鼠伤寒沙门氏菌的抗菌作用模式。

由 Lactococcus garvieae LG34 生产的 Garviecin LG34 对革兰氏阳性菌和革兰氏阴性菌均具有广谱抗菌活性。本研究旨在阐明蒜维菌素 LG34 对革兰氏阴性菌鼠伤寒杆菌的抗菌作用模式。为了确定细菌素抗菌模式实验的浓度，确定了加维素 LG34 对伤寒杆菌 CICC21484 的最低抗菌浓度为 0.25 mg/ml。Garviecin LG34 降低了伤寒杆菌 CICC21484 的存活数量，其抗菌活性与剂量和时间有关。Garviecin LG34 会导致伤寒杆菌 CICC21484 的跨膜电位消散、细胞外电导率上升、在 260 纳米波长下的紫外线吸收物质和 LDH 水平下降。扫描电子显微镜结果表明，加维素 LG34 会导致伤寒杆菌细胞剧烈变形和破碎，包括鞭毛脱落、表面形成孔隙，甚至完全破裂。此外，加维素 LG34 还会降低细胞内的 ATP 水平。研究结果表明，蒜维素 LG34 能破坏伤寒杆菌的细胞结构，增加其膜的通透性，因此可用作治疗由革兰氏阴性伤寒杆菌引起的食源性疾病和沙门氏菌病的生物防腐剂。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.