Transcriptome analysis reveals candidate genes for different root types of alfalfa (Medicago sativa) after water stress induced by PEG-6000

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2024-08-05 DOI:10.1186/s40538-024-00640-7

Kun Wang, Li-Li Nan, Jing Xia, Yu-heng Yao, Jiao Cheng, Jing-ru Chen

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引用次数: 0

Abstract

Background

We aimed to gain insight into the response mechanism of alfalfa (Medicago sativa) to drought stress by recognizing and analyzing drought-responsive genes in the roots of different root types of alfalfa. The rhizomatous-rooted M. sativa cv.‘Qingshui’ (QS), tap-rooted M. sativa cv.‘Longdong’ (LD), and creeping-rooted M. varia cv. ‘Gongnong No. 4’ (GN) were used to analyze the transcriptome information and physiological characteristics of the root systems of the cultivars under simulated drought stress using PEG-6000.

Results

It was found that aridity caused a significant increase in the content of osmotic stress substances and antioxidant enzyme activity. The content of malondialdehyde (MDA) in QS was lower than that in LD and GN under moisture stress, indicating a stronger accumulation capacity of osmotic regulatory substances. Based on sequencing results, 14,475, 9336, and 9243 upregulated DEGs from QS, LD, and GN were annotated into 26, 29, and 28 transcription factor families, respectively. QS showed more DEGs than LD and GN. KEGG enrichment analysis identified that DEGs were significantly enriched in metabolic pathways such as amino acid biosynthesis, phenylpropanoid biosynthesis, plant hormone signaling transduction, and MAPK pathways. This suggests a strong correlation between these pathways and drought stress. The results also show that genes associated with ABA hormone signaling (MS. gene93372, MS. gene072046, and MS. gene012975) are crucial for plant’s adaptation to drought stress.

Conclusions

These genes, such as serine/threonine protein kinases and abscisic acid receptors, play a crucial role in plant hormone signaling and MAPK pathways. They could serve as potential candidate genes for drought resistance research in alfalfa, providing a molecular foundation for studying drought resistance.

Graphical Abstract

查看原文本刊更多论文

转录组分析揭示了 PEG-6000 诱导水分胁迫后紫花苜蓿（Medicago sativa）不同根类型的候选基因

我们的目的是通过识别和分析不同根系类型紫花苜蓿根中的干旱响应基因，了解紫花苜蓿（Medicago sativa）对干旱胁迫的响应机制。利用PEG-6000分析了根瘤根M. sativa cv.'清水'（QS）、直根M. sativa cv.'陇东'（LD）和匍匐根M. varia cv.'工农4号'（GN）在模拟干旱胁迫下根系的转录组信息和生理特征。结果发现，干旱会导致渗透胁迫物质含量和抗氧化酶活性显著增加。在水分胁迫下，QS 的丙二醛（MDA）含量低于 LD 和 GN，表明其渗透调节物质的积累能力更强。根据测序结果，QS、LD 和 GN 的 14475、9336 和 9243 个上调 DEGs 分别被注释为 26、29 和 28 个转录因子家族。与 LD 和 GN 相比，QS 显示了更多的 DEGs。KEGG 富集分析发现，DEGs 在氨基酸生物合成、苯丙类化合物生物合成、植物激素信号转导和 MAPK 通路等代谢途径中明显富集。这表明这些途径与干旱胁迫之间存在密切联系。结果还显示，与 ABA 激素信号转导相关的基因（MS. gene93372、MS. gene072046 和 MS. gene012975）对植物适应干旱胁迫至关重要。这些基因，如丝氨酸/苏氨酸蛋白激酶和脱落酸受体，在植物激素信号转导和 MAPK 途径中发挥着重要作用。它们可以作为苜蓿抗旱研究的潜在候选基因，为抗旱研究提供分子基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.