Design and implementation of a lab-on-a-chip for assisted reproductive technologies.

IF 2.2 4区 工程技术 Q3 PHARMACOLOGY & PHARMACY
Bioimpacts Pub Date : 2024-01-01 Epub Date: 2023-12-10 DOI:10.34172/bi.2023.28902
Firooz Safaefar, Javad Karamdel, Hadi Veladi, Masoud Maleki
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引用次数: 0

Abstract

Introduction: The microfluidic device is highly optimized to remove oocytes from the cumulus-corona cell mass surrounding them. Additionally, it effectively captures and immobilizes the oocytes, aiding in assessing their quality and facilitating the injection of sperm into the oocyte. In this study, a novel microfluidic chip was designed and manufactured using conventional soft lithography methods.

Methods: This research proposes the utilization of a microfluidic chip as a substitute for the conventional manual procedures involved in oocyte denudation, trapping, and immobilization. The microfluidic chip was modeled and simulated using COMSOL Multiphysics® 5.2 software to optimize and enhance its design and performance. The microfluidic chip was fabricated using conventional injection molding techniques on a polydimethylsiloxane substrate by employing soft lithography methods.

Results: A hydrostatic force was applied to guide the oocyte through predetermined pathways to eliminate the cumulus cells surrounding the oocyte. The oocyte was subsequently confined within the designated trap region by utilizing hydraulic resistance along the paths and immobilized by applying vacuum force.

Conclusion: The application of this chip necessitates a lower level of operator expertise compared to enzymatic and mechanical techniques. Moreover, it is feasible to continuously monitor the oocyte's state throughout the procedure. There is a reduced need for cultural media compared to more standard approaches.

辅助生殖技术芯片实验室的设计与实施。
简介微流体设备经过高度优化,可将卵母细胞从其周围的积液-副细胞团中取出。此外,它还能有效捕获并固定卵母细胞,有助于评估卵母细胞的质量,并方便将精子注入卵母细胞。本研究采用传统的软光刻方法设计并制造了一种新型微流控芯片:本研究提出利用微流体芯片替代传统的人工去核、捕获和固定卵母细胞的方法。使用 COMSOL Multiphysics® 5.2 软件对微流体芯片进行建模和模拟,以优化和提高其设计和性能。采用软光刻方法,在聚二甲基硅氧烷基底上使用传统注塑技术制作了微流控芯片:应用静水压力引导卵母细胞通过预定路径,消除卵母细胞周围的积层细胞。随后,利用路径上的液压阻力将卵母细胞限制在指定的捕获区域内,并通过施加真空力将其固定:结论:与酶解技术和机械技术相比,这种芯片的应用对操作人员的专业技能要求较低。此外,在整个过程中持续监测卵母细胞的状态也是可行的。与标准方法相比,对培养基的需求也有所减少。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Bioimpacts
Bioimpacts Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
4.80
自引率
7.70%
发文量
36
审稿时长
5 weeks
期刊介绍: BioImpacts (BI) is a peer-reviewed multidisciplinary international journal, covering original research articles, reviews, commentaries, hypotheses, methodologies, and visions/reflections dealing with all aspects of biological and biomedical researches at molecular, cellular, functional and translational dimensions.
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