Encapsulation of Lactobacillus acidophilus or Bifidobacterium longum Using Whey Protein Concentrate and Fructans as Wall Materials: Properties, Stability, and Viability in Simulated Gastrointestinal Tests

IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY

ACS food science & technology Pub Date : 2024-08-02 DOI:10.1021/acsfoodscitech.4c00235

Naida Juárez-Trujillo, Audry Peredo-Lovillo, Fidel Martínez-Gutiérrez, Beatriz Pérez-Armendáriz, Rosa I. Ortiz-Basurto, Maribel Jiménez- Fernández

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Abstract

Whey protein concentrate (WPC) and native agave fructans (NAFs) were used in the microencapsulation process of Lactobacillus acidophilus La-14 or Bifidobacterium longum subsp. infantis. Two treatments were carried out: 1. double emulsion-WPC-NAF and 2. WPC-NAF, both treatments were spray dried. Physicochemical, flow, and reconstitution properties, as well as thermal and microstructural stability were evaluated in microcapsules. Encapsulation efficiency after storage (one year, 4 °C) was determined under simulated gastrointestinal tests. The double emulsion-WPC-NAF showed an encapsulation efficiency >76%, whereas this efficiency reached up to 95% in dried microcapsules. Both treatments showed viability of 8 and 9 log CFU/g after storage and in vitro digestion, respectively. Also, the formation of structural WPC-NAF complexes, related to thermal and structural stability, was observed in microcapsules of double emulsion-WPC-NAF. Hence, microcapsules made from double emulsion of WPC-NAF showed greater physicochemical, structural, and microbial stability, suggesting their use as potentially functional food additive.

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以浓缩乳清蛋白和果聚糖为壁材封装嗜酸乳杆菌或长双歧杆菌：模拟胃肠道试验中的特性、稳定性和活力

在嗜酸乳杆菌 La-14 或长双歧杆菌亚种的微胶囊化过程中使用了浓缩乳清蛋白（WPC）和原生龙舌兰果聚糖（NAFs）。共进行了两种处理1. 双乳液-WPC-NAF；2.WPC-NAF，两种处理均采用喷雾干燥。对微胶囊的理化、流动和重组特性以及热稳定性和微结构稳定性进行了评估。在模拟胃肠道试验中测定了储存（一年，4 °C）后的封装效率。双乳液-WPC-NAF 的封装效率为 76%，而干燥微胶囊的封装效率则高达 95%。两种处理方法在储存和体外消化后的存活率分别为 8 和 9 log CFU/g。此外，在双乳液-WPC-NAF 的微胶囊中还观察到 WPC-NAF 结构复合物的形成，这与热稳定性和结构稳定性有关。因此，由 WPC-NAF 双乳液制成的微胶囊显示出更高的理化、结构和微生物稳定性，建议将其用作潜在的功能性食品添加剂。

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来源期刊

ACS food science & technology

CiteScore

3.30

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0.00%

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