Recycling potato waste for the production of blue pigments by Streptomyces lydicus PM7 through submerged fermentation

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Chemical and Biological Technologies in Agriculture Pub Date : 2024-07-24 DOI:10.1186/s40538-024-00612-x

Álvaro Astudillo, Emilio Hormazábal, Andrés Quiroz, Olga Rubilar, Gabriela Briceño, Roberto Abdala, Claudio Lamilla, María Cristina Diez, Heidi Schalchli

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Abstract

Background

Discarded potato is the most abundant potato waste and represents a worldwide disposal problem to the potato industry. This agricultural waste contains valuable nutrients that could be used as substrate to obtain diverse high value-added microbial products, such as biopigments. The aim of this work was to evaluate the use of discarded potato as a sole substrate source for producing blue pigments by Streptomyces lydicus PM7 through submerged fermentation.

Results

Initially, the traditional culture medium ISP2 was established as suitable for inoculum preparation, as it allowed high growth rates and consumption of ~ 75% reducing sugar, leading to 1.3 g L⁻¹ dry biomass at 72 h of incubation. The formulated discarded potato broth (DPB) medium was evaluated together with five other traditional liquid culture media (potato dextrose broth, ISP2, ISP3, ISP4, and ISP5) for producing blue pigments by S. lydicus PM7. The highest blue pigment production was obtained by using DPB medium, reaching ~ 0.97 g L⁻¹, followed by ISP5 (~ 0.36 g L⁻¹). In terms of evaluating the concentration of discarded potato powder, the highest concentration of blue pigments was obtained with 16 g L⁻¹, compared to concentrations of 4, 8, and 32 g L⁻¹. In general, a notable increase in total proteins (~ 14 g L⁻¹ in biomass; ~ 8 g L⁻¹ in medium) and reducing sugars (~ 5 g L⁻¹) on the fifth day of DPB fermentation was observed, at which time the production of blue pigments began. These data proved that S. lydicus PM7 is able to degrade potato wastes during submerged fermentation and to direct metabolism towards the formation of biopigments. Chromatographic analysis revealed that the main blue pigment produced by new strain in this complex medium is actinorhodin.

Conclusions

Discarded potato favored the production of blue pigments by S. lydicus PM7 under submerged fermentation, leading to final product concentration almost three times higher than others traditional Streptomyces culture media. To the best of our knowledge, this is the first report on the production of actinorhodin by the specie S. lydicus, as well as on this pigment synthesis based on an agricultural waste as a sole nutrient source for fermentation process. The findings showed that potato waste could be a potential byproduct for replacement of commercial culture media using for this same purpose.

Graphical Abstract

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通过浸没式发酵回收马铃薯废料，利用莱迪克链霉菌 PM7 生产蓝色色素

废弃马铃薯是最丰富的马铃薯废弃物，也是马铃薯产业面临的一个全球性处置问题。这种农业废弃物含有宝贵的营养物质，可用作底物来获得多种高附加值的微生物产品，如生物色素。这项工作的目的是评估利用废弃马铃薯作为唯一基质来源，通过浸没式发酵由吕氏链霉菌 PM7 生产蓝色色素的情况。最初，传统培养基 ISP2 被确定为适合接种物制备的培养基，因为它允许高生长率和消耗 ~ 75% 的还原糖，从而在培养 72 小时后产生 1.3 g L-1 的干生物量。将配制好的废弃马铃薯肉汤（DPB）培养基与其他五种传统液体培养基（马铃薯葡萄糖肉汤、ISP2、ISP3、ISP4 和 ISP5）一起进行了评估，以确定赖德氏菌 PM7 是否能产生蓝色素。使用 DPB 培养基产生的蓝色色素最高，达到 ~ 0.97 g L-1，其次是 ISP5（~ 0.36 g L-1）。在评估废弃马铃薯粉末的浓度时，16 g L-1 的蓝色色素浓度最高，而 4、8 和 32 g L-1 的浓度最低。总的来说，在 DPB 发酵的第五天，观察到总蛋白（生物量约 14 g L-1；培养基约 8 g L-1）和还原糖（约 5 g L-1）显著增加，此时开始产生蓝色色素。这些数据证明，S. lydicus PM7 能够在浸没发酵过程中降解马铃薯废弃物，并将新陈代谢导向生物色素的形成。色谱分析显示，新菌株在这种复合培养基中产生的主要蓝色色素是放线菌素。丢弃的马铃薯有利于 S. lydicus PM7 在浸没发酵条件下产生蓝色色素，其最终产品浓度几乎是其他传统链霉菌培养基的三倍。据我们所知，这是第一份关于莱迪氏菌（S. lydicus）生产放线菌素的报告，也是第一份关于以农业废弃物作为发酵过程唯一营养源合成色素的报告。研究结果表明，马铃薯废弃物可以作为一种潜在的副产品，替代用于相同目的的商业培养基。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.