Isolation, identification, and biological characterization of bacterial endophytes isolated from Gunnera perpensa L.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae056

Siphiwe Godfrey Mahlangu, Nodumo Zulu, Mahloro Hope Serepa-Dlamini, Siew Leng Tai

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引用次数: 0

Abstract

In the present study, eleven endophytic bacterial strains, Herbaspirillum sp. (GP-SGM1, GP-SGM2, GP-SGM3, and GP-SGM11), Pseudomonas sp. (GP-SGM4, GP-SGM5), Novosphingobium sp. GP-SGM6, Chryseobacterium sp. GP-SGM7, Labedella sp. GP-SGM8, Brevibacterium sp. GP-SGM9, and Pseudomonas sp. GP-SGM10, were isolated from the rhizomes of Gunnera perpensa L. The growth kinetics, assessed through maximum growth rates (μmax) and optical density (OD) values, revealed that GP-SGM7 exhibited highest μmax values of 0.33 ± 0.01 hours (h)-1 with an OD of 4.20 ± 0.04. In contrast, GP-SGM11 exhibited the lowest μmax of 0.12 ± 0.05 h-1 and the smallest OD of 1.50 ± 0.00. In addition, the endophyte crude extracts were tested for antibacterial activity against five pathogenic strains using the disk diffusion method, with GP-SGM7 crude extracts exhibiting promising antibacterial activity against Klebsiella pneumoniae and Staphylococcus aureus. Antioxidant activity was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays. The crude extracts of GP-SGM1, GP-SGM7, GP-SGM9, and GP-SGM10 were the most effective at scavenging DPPH radicals, with GP-SGM7 also exhibiting a high FRAP value of 0.54 ± 0.01. These findings emphasize the therapeutic potential of endophytic bacteria from G. perpensa L. in addressing skin-related issues, including bacterial infections and free radicals.

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从Gunnera Perpensa L.中分离的细菌内生菌的分离、鉴定和生物学特性分析

GP-SGM1, GP-SGM2, GP-SGM3, GP-SGM11), Pseudomonas sp.通过最大生长速率（µmax）和光密度（OD）值评估的生长动力学表明，GP-SGM7 的最大µmax 值为 0.33 ± 0.01 hr-1，光密度为 4.20 ± 0.04。相比之下，GP-SGM11 的 µmax 值最低，为 0.12 ± 0.05 hr-1，OD 值最小，为 1.50 ± 0.00。此外，还使用盘扩散法测试了内生菌粗萃取物对五种病原菌株的抗菌活性，其中 GP-SGM7 粗萃取物对肺炎克雷伯氏菌和金黄色葡萄球菌具有良好的抗菌活性。抗氧化活性通过 DPPH（2, 2-二苯基-1-苦基肼）和 FRAP（铁还原抗氧化能力）检测法进行测定。GP-SGM1、GP-SGM7、GP-SGM9 和 GP-SGM10 的粗提取物在清除 DPPH 自由基方面最为有效，GP-SGM7 的 FRAP 值也高达 0.54 ± 0.01。这些发现强调了Gunnera perpensa L.内生细菌在解决皮肤相关问题（包括细菌感染和自由基）方面的治疗潜力。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.