Effect of SHAM on the activity of coumoxystrobin against Phytophthora litchii

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences
Suyue Jing, Fadi Zhu, Xiaodong Wen, Jing Zhang, Gang Feng
{"title":"Effect of SHAM on the activity of coumoxystrobin against Phytophthora litchii","authors":"Suyue Jing, Fadi Zhu, Xiaodong Wen, Jing Zhang, Gang Feng","doi":"10.1007/s40858-024-00657-1","DOIUrl":null,"url":null,"abstract":"<p>Litchi downy blight, caused by <i>Phytophthora litchii</i>, presents significant challenges to litchi production, storage, and transportation. Previous studies have shown that coumoxystrobin exhibits effective inhibitory activity against <i>P</i>. <i>litchii</i>. Salicylhydroxamic acid (SHAM), an alternative respiratory pathway inhibitor, is commonly used to evaluate the efficacy of cytochrome respiratory pathway inhibitor like coumoxystrobin against fungal phytopathogens <i>in vitro</i>. In this study, the toxicity of SHAM on various developmental stages of <i>P</i>. <i>litchii</i>, including mycelial growth, sporangial germination, zoospore release, and cystospore germination, was assessed. The EC<sub>50</sub> values for SHAM were determined as 166.72, 150.69, 333.97, and 240.91 μg/mL, respectively. Subsequently, the activity of coumoxystrobin against <i>P</i>. <i>litchii</i> was assessed in the presence of SHAM at a concentration of 50 μg/mL, which showed slight inhibition below 20% for all four developmental stages. The addition of SHAM significantly improved the inhibitory activity of coumoxystrobin against <i>P</i>. <i>litchii</i> at different stages, with reductions in EC<sub>50</sub> values ranging from 7.55- to 122.92-fold. Moreover, respiration assays revealed that a concentration of 5 μg/mL coumoxystrobin inhibited <i>P</i>. <i>litchii</i> mycelial respiration to a lesser extent compared to the combined effect of coumoxystrobin and SHAM. SHAM also enhanced the control efficacy of coumoxystrobin against phytophthora blight development on litchi leaves. Previously, we reported that coumoxystrobin effectively controls postharvest downy mildew on litchi fruit. Consequently, coumoxystrobin holds promise as an agent for litchi downy blight control in the field and after harvest. Furthermore, similar to previous studies, SHAM, an alternative oxidase (AOX) inhibitor, was found to significantly enhance the activity of the two aforementioned QoI fungicides against <i>P</i>. <i>litchii</i>, both <i>in vitro</i> and <i>in vivo</i>. This suggests that further exploration of AOX inhibitors and the role of AOX in plant diseases could contribute to the rational use of QoI fungicides and improve control efficiency for plant diseases.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00657-1","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

Litchi downy blight, caused by Phytophthora litchii, presents significant challenges to litchi production, storage, and transportation. Previous studies have shown that coumoxystrobin exhibits effective inhibitory activity against P. litchii. Salicylhydroxamic acid (SHAM), an alternative respiratory pathway inhibitor, is commonly used to evaluate the efficacy of cytochrome respiratory pathway inhibitor like coumoxystrobin against fungal phytopathogens in vitro. In this study, the toxicity of SHAM on various developmental stages of P. litchii, including mycelial growth, sporangial germination, zoospore release, and cystospore germination, was assessed. The EC50 values for SHAM were determined as 166.72, 150.69, 333.97, and 240.91 μg/mL, respectively. Subsequently, the activity of coumoxystrobin against P. litchii was assessed in the presence of SHAM at a concentration of 50 μg/mL, which showed slight inhibition below 20% for all four developmental stages. The addition of SHAM significantly improved the inhibitory activity of coumoxystrobin against P. litchii at different stages, with reductions in EC50 values ranging from 7.55- to 122.92-fold. Moreover, respiration assays revealed that a concentration of 5 μg/mL coumoxystrobin inhibited P. litchii mycelial respiration to a lesser extent compared to the combined effect of coumoxystrobin and SHAM. SHAM also enhanced the control efficacy of coumoxystrobin against phytophthora blight development on litchi leaves. Previously, we reported that coumoxystrobin effectively controls postharvest downy mildew on litchi fruit. Consequently, coumoxystrobin holds promise as an agent for litchi downy blight control in the field and after harvest. Furthermore, similar to previous studies, SHAM, an alternative oxidase (AOX) inhibitor, was found to significantly enhance the activity of the two aforementioned QoI fungicides against P. litchii, both in vitro and in vivo. This suggests that further exploration of AOX inhibitors and the role of AOX in plant diseases could contribute to the rational use of QoI fungicides and improve control efficiency for plant diseases.

Abstract Image

SHAM 对唑菌酰胺(coumoxystrobin)抗荔枝疫霉活性的影响
由荔枝疫霉(Phytophthora litchii)引起的荔枝霜霉病给荔枝的生产、储藏和运输带来了巨大挑战。先前的研究表明,唑啉草酯(coumoxystrobin)对荔枝疫霉具有有效的抑制活性。水杨羟肟酸(SHAM)是一种可供选择的呼吸途径抑制剂,常用于体外评估细胞色素呼吸途径抑制剂(如唑啉草酯)对真菌植物病原体的功效。本研究评估了 SHAM 对荔枝角菌不同发育阶段的毒性,包括菌丝生长、孢子囊萌发、子囊孢子释放和子囊孢子萌发。经测定,SHAM 的 EC50 值分别为 166.72、150.69、333.97 和 240.91 μg/mL。随后,在 50 μg/mL 浓度的 SHAM 存在下,评估了唑啉草酯对荔枝角的活性,结果表明,在所有四个发育阶段,唑啉草酯对荔枝角的抑制率均低于 20%。加入 SHAM 后,唑啉草酯对不同发育阶段的荔枝核的抑制活性明显提高,EC50 值降低了 7.55 至 122.92 倍。此外,呼吸作用试验表明,与唑啉草酯和 SHAM 的联合作用相比,浓度为 5 μg/mL 的唑啉草酯对 P. litchii 菌丝呼吸作用的抑制程度较低。SHAM还增强了唑啉草酯对荔枝叶片疫霉病的防治效果。此前,我们曾报道唑啉草酯能有效控制荔枝果采后霜霉病。因此,coumoxystrobin 有希望成为田间和采后荔枝霜霉病的防治剂。此外,与之前的研究类似,研究发现替代氧化酶(AOX)抑制剂 SHAM 在体外和体内都能显著增强上述两种 QoI 杀菌剂对荔枝霜霉病的活性。这表明,进一步探索 AOX 抑制剂和 AOX 在植物病害中的作用有助于合理使用 QoI 杀菌剂,提高植物病害的防治效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信