Synthesis of pinolenic acid-enriched triacylglycerol from pine nut oil via a two-step consecutive enzyme reaction: Comparison of acyl donors

IF 1.9 4区农林科学 Q3 CHEMISTRY, APPLIED

Journal of the American Oil Chemists Society Pub Date : 2024-07-17 DOI:10.1002/aocs.12881

Yu Jin Lee, Mi Soon Park, Byung Hee Kim, Yangha Kim, In-Hwan Kim

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引用次数: 0

Abstract

Pinolenic acid (PLA) is a plant-origin Δ5-unsaturated polymethylene-interrupted fatty acid that provides several beneficial health effects to the human body. A two-step lipase-catalyzed reaction was carried out to synthesize PLA-enriched triacylglycerol (TAG) with pine nut oil (PNO) in the present study. In the first step, PLA was efficiently enriched from an initial value of 15–43 mol% in ethyl ester of the reaction mixture after Lipozyme 435-catalyzed ethanolysis with PNO. In the second step, PLA-enriched TAG was efficiently synthesized with the fatty acid form via Lipozyme 435-catalyzed esterification at all temperatures when both ethyl ester form and fatty acid form were compared as acyl donors. The effect of vacuum on the synthesis of PLA-enriched TAG with glycerol and PLA-enriched fatty acid was studied. The optimum temperature and vacuum were 60°C, and 50 mmHg, respectively. A maximum TAG conversion of approximately 95% was achieved after 12 h under the optimum conditions.

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通过两步连续酶反应从松子油中合成富含松油酸的三酰甘油：酰基供体的比较

松油酸（PLA）是一种源于植物的Δ5-不饱和多亚甲基间断脂肪酸，对人体有多种有益的保健作用。本研究采用两步脂肪酶催化反应，用松子油（PNO）合成富含聚乳酸的三酰甘油（TAG）。在第一步中，经 Lipozyme 435 催化乙醇分解松子油后，聚乳酸在反应混合物乙酯中的含量从初始值的 15-43 摩尔%有效富集。在第二步中，通过 Lipozyme 435 催化的酯化反应，以乙酯和脂肪酸作为酰基供体，在所有温度下都能高效合成富含聚乳酸的 TAG。研究了真空对富含聚乳酸的 TAG 与甘油和富含聚乳酸的脂肪酸合成的影响。最佳温度和真空度分别为 60°C 和 50 mmHg。在最佳条件下，12 小时后 TAG 的最大转化率约为 95%。

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来源期刊

Journal of the American Oil Chemists Society 工程技术-食品科技

CiteScore

4.10

自引率

5.00%

发文量

审稿时长

2.4 months

期刊介绍： The Journal of the American Oil Chemists’ Society (JAOCS) is an international peer-reviewed journal that publishes significant original scientific research and technological advances on fats, oils, oilseed proteins, and related materials through original research articles, invited reviews, short communications, and letters to the editor. We seek to publish reports that will significantly advance scientific understanding through hypothesis driven research, innovations, and important new information pertaining to analysis, properties, processing, products, and applications of these food and industrial resources. Breakthroughs in food science and technology, biotechnology (including genomics, biomechanisms, biocatalysis and bioprocessing), and industrial products and applications are particularly appropriate. JAOCS also considers reports on the lipid composition of new, unique, and traditional sources of lipids that definitively address a research hypothesis and advances scientific understanding. However, the genus and species of the source must be verified by appropriate means of classification. In addition, the GPS location of the harvested materials and seed or vegetative samples should be deposited in an accredited germplasm repository. Compositional data suitable for Original Research Articles must embody replicated estimate of tissue constituents, such as oil, protein, carbohydrate, fatty acid, phospholipid, tocopherol, sterol, and carotenoid compositions. Other components unique to the specific plant or animal source may be reported. Furthermore, lipid composition papers should incorporate elements of yeartoyear, environmental, and/ or cultivar variations through use of appropriate statistical analyses.