The Allergen Profile of Two Edible Insect Species—Acheta domesticus and Hermetia illucens

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research Pub Date : 2024-07-18 DOI:10.1002/mnfr.202300811

Shaymaviswanathan Karnaneedi, Elecia B. Johnston, Utpal Bose, Angéla Juhász, James A. Broadbent, Thimo Ruethers, Emily M. Jerry, Sandip D. Kamath, Vachiranee Limviphuvadh, Sally Stockwell, Keren Byrne, Dean Clarke, Michelle L. Colgrave, Sebastian Maurer-Stroh, Andreas L. Lopata

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Abstract

Scope

Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need to feed the growing population. Tropomyosin is the main insect allergen; however, additional potential allergens are not well characterized and the impact of extraction procedures on immunological reactivity is unknown.

Methods and results

Proteins from different commercial food products derived from cricket (Acheta domesticus) and black soldier fly (BSF) (Hermetia illucens) are extracted using five different extraction buffers. The proteins are analyzed by SDS-PAGE and immunoblotting using allergen-specific antibodies and crustacean allergic patient sera. IgE binding bands are analyzed by mass spectrometry as well as the complete allergen profile of all 30 extracts. Urea-based buffers are most efficient in extracting insect allergens. Shrimp-specific antibody cross-reactivity to tropomyosin from cricket and BSF indicates high sequence and structural similarity between shrimp and insects. Additional unique allergens are identified in both species, including hemocyanin, vitellogenin, HSP20, apolipophorin-III, and chitin-binding protein.

Conclusions

Identifying potential allergenic proteins and their isoforms in cricket and BSF requires specific extraction approaches using urea-based methods. While tropomyosin is the most abundant and immunoreactive allergen, seven unique allergens are identified, highlighting the need for insect species-specific allergen detection in food products.

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两种可食用昆虫--家蝇和白头翁的过敏原概况

范围：可食用昆虫蛋白作为一种可替代的可持续食物来源，越来越多地被采用，以满足世界上不断增长的人口对食物的需求。托布霉素是主要的昆虫过敏原；然而，其他潜在过敏原的特征还不明确，提取程序对免疫反应性的影响也不清楚：使用五种不同的提取缓冲液提取了来自蟋蟀（Acheta domesticus）和黑兵蝇（BSF）（Hermetia illucens）的不同商业食品中的蛋白质。使用过敏原特异性抗体和甲壳类过敏患者血清对蛋白质进行 SDS-PAGE 和免疫印迹分析。通过质谱分析 IgE 结合带以及所有 30 种提取物的完整过敏原图谱。基于尿素的缓冲液提取昆虫过敏原的效率最高。虾特异性抗体与蟋蟀和 BSF 的肌球蛋白的交叉反应表明，虾与昆虫的序列和结构高度相似。在这两个物种中还发现了其他独特的过敏原，包括血蓝蛋白、卵黄素、HSP20、apolipophorin-III和几丁质结合蛋白：鉴定蟋蟀和BSF中潜在的致敏蛋白质及其同工形式需要使用基于尿素的特定提取方法。虽然肌球蛋白是含量最高、免疫反应最强的过敏原，但也发现了七种独特的过敏原，这凸显了在食品中检测昆虫物种特异性过敏原的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Nutrition & Food Research 工程技术-食品科技

CiteScore

8.70

自引率

1.90%

发文量

250

审稿时长

1.7 months

期刊介绍： Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.