Fructooligosaccharides Ameliorate Renal Injury and Dysfunction Through the Modulation of Gut Dysbiosis, Inhibition of Renal Inflammation, Oxidative Stress, Fibrosis, and Improve Organic Anion Transporter 3 Function in an Obese Rat Model

IF 4.5 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Molecular Nutrition & Food Research Pub Date : 2024-07-17 DOI:10.1002/mnfr.202400191

Nattavadee Pengrattanachot, Laongdao Thongnak, Sasivimon Promsan, Nichakorn Phengpol, Prempree Sutthasupha, Jiraporn Tocharus, Anusorn Lungkaphin

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Abstract

Scope

High-fat diet (HFD) consumption causes obesity and gut dysbiosis which induces kidney injury. It has been reported that prebiotics improve gut dysbiosis and insulin sensitivity and decelerate the progression of kidney disease. This study investigates the impact of fructooligosaccharides (FOS) on renoprotection and the prevention of gut dysbiosis and intestinal barrier injury in obese rats.

Methods and results

Wistar rats are treated with HFD for 16 weeks. Then, the HFD fed rats (HF) are given FOS 1 g day⁻¹ (HFFOS1), 2 g day⁻¹ (HFFOS2), or metformin 30 mg kg⁻¹ day⁻¹ (HFMET), by intragastric feeding for 8 weeks. Blood, urine, feces, kidney, and intestine are collected to determine the metabolic changes, gut dysbiosis, and the expression of proteins involved in kidney and intestinal injury. FOS can attenuate insulin resistance and hypercholesterolemia concomitant with the inhibition of renal inflammation, oxidative stress, fibrosis, and apoptosis, which are related to the deceleration of the overexpression of renal Toll-like receptor 4 (TLR4) and NADPH oxidase (NOX4). Moreover, FOS shows a greater efficacy than metformin in the reduction of the intestinal injury and loss of tight junction proteins induced by HFD.

Conclusion

FOS may be used as a supplement for therapeutic purposes in an obese condition to improve intestinal integrity and prevent renal complications.

Abstract Image

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果寡糖通过调节肠道菌群失调、抑制肾脏炎症、氧化应激和纤维化以及改善肥胖大鼠模型中有机阴离子转运体 3 的功能，改善肾脏损伤和功能障碍。

范围高脂饮食（HFD）会导致肥胖和肠道菌群失调，从而诱发肾损伤。据报道，益生元可改善肠道菌群失调和胰岛素敏感性，并减缓肾脏疾病的进展。本研究探讨了果寡糖（FOS）对肥胖大鼠肾脏保护和预防肠道菌群失调及肠道屏障损伤的影响：对 Wistar 大鼠进行为期 16 周的 HFD 治疗。然后，通过胃内喂食给予 FOS 1 克/天-1（HFFOS1）、2 克/天-1（HFFOS2）或二甲双胍 30 毫克/公斤-1 天-1（HFMET），连续喂食 8 周。收集血液、尿液、粪便、肾脏和肠道，以确定代谢变化、肠道菌群失调以及参与肾脏和肠道损伤的蛋白质的表达。FOS 可减轻胰岛素抵抗和高胆固醇血症，同时抑制肾脏炎症、氧化应激、纤维化和细胞凋亡，这与减缓肾脏 Toll 样受体 4（TLR4）和 NADPH 氧化酶（NOX4）的过度表达有关。此外，与二甲双胍相比，FOS 在减少高氟酸膳食引起的肠道损伤和紧密连接蛋白损失方面表现出更大的功效：结论：FOS 可用作肥胖症患者的治疗补充剂，以改善肠道完整性并预防肾脏并发症。

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来源期刊

Molecular Nutrition & Food Research 工程技术-食品科技

CiteScore

8.70

自引率

1.90%

发文量

250

审稿时长

1.7 months

期刊介绍： Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.