Effects of catechin on the malignant biological behavior of gastric cancer cells through the PI3K/Akt signaling pathway

IF 3.3 3区医学 Q2 PHARMACOLOGY & PHARMACY

Toxicology and applied pharmacology Pub Date : 2024-07-14 DOI:10.1016/j.taap.2024.117036

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引用次数: 0

Abstract

Catechin is a kind of flavonoids, mainly derived from the plant Camellia sinensis. It has a strong antioxidant effect, and it also has significant therapeutic effects on anti-cancer, anti-diabetes, and anti-infection. This study was intended to look at how catechin affected the malignant biological activity of gastric cancer cells. We used databases to predict the targets of catechin and the pathogenic targets of gastric cancer. Venn diagram was used to find the intersection genes, the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were performed on intersection genes. Using the STRING database, the Protein-Protein Interaction (PPI) network was built. The top 8 genes were screened by Cytoscape 3.9.1, then their binding was verified by molecular docking. The proliferation ability, cell cycle, apoptosis and migration of gastric cancer cells were detected, as well as the protein expression levels of PI3K, p-AKT, and AKT and the mRNA expression levels of AKT1, VEGFA, EGFR, HRAS, and HSP90AA1 in gastric cancer cells. Our research revealed that different concentrations of catechin could effectively inhibit the proliferation and migration of gastric cancer cells, regulate the cell cycle, and promote the death of these cells, and it's possible that the PI3K/Akt pathway was crucial in mediating this impact. Moreover, adding the PI3K/Akt pathway agonist significantly reduced the promoting effect of catechin on the apoptosis of gastric cancer cells. This study suggested that catechin was a potential drug for the treatment of gastric cancer.

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儿茶素通过 PI3K/Akt 信号通路对胃癌细胞恶性生物学行为的影响

儿茶素是一种黄酮类化合物，主要来源于植物山茶。它具有很强的抗氧化作用，在抗癌、抗糖尿病、抗感染等方面也有显著的治疗效果。本研究旨在探讨儿茶素如何影响胃癌细胞的恶性生物活性。我们利用数据库预测了儿茶素的靶点和胃癌的致病靶点。我们利用维恩图找到了交叉基因，并对交叉基因进行了京都基因组百科全书（KEGG）和基因本体（GO）富集分析。利用 STRING 数据库，建立了蛋白质-蛋白质相互作用（PPI）网络。通过 Cytoscape 3.9.1 筛选出前 8 个基因，并通过分子对接验证了它们之间的结合。我们检测了胃癌细胞的增殖能力、细胞周期、凋亡和迁移，以及胃癌细胞中 PI3K、p-AKT 和 AKT 的蛋白表达水平和 AKT1、VEGFA、EGFR、HRAS 和 HSP90AA1 的 mRNA 表达水平。我们的研究发现，不同浓度的儿茶素能有效抑制胃癌细胞的增殖和迁移，调节细胞周期，并促进这些细胞的死亡，而PI3K/Akt通路可能是介导这种影响的关键。此外，添加 PI3K/Akt 激动剂会明显降低儿茶素对胃癌细胞凋亡的促进作用。这项研究表明，儿茶素是一种治疗胃癌的潜在药物。

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来源期刊

Toxicology and applied pharmacology 医学-毒理学

CiteScore

6.80

自引率

2.60%

发文量

309

审稿时长

32 days

期刊介绍： Toxicology and Applied Pharmacology publishes original scientific research of relevance to animals or humans pertaining to the action of chemicals, drugs, or chemically-defined natural products. Regular articles address mechanistic approaches to physiological, pharmacologic, biochemical, cellular, or molecular understanding of toxicologic/pathologic lesions and to methods used to describe these responses. Safety Science articles address outstanding state-of-the-art preclinical and human translational characterization of drug and chemical safety employing cutting-edge science. Highly significant Regulatory Safety Science articles will also be considered in this category. Papers concerned with alternatives to the use of experimental animals are encouraged. Short articles report on high impact studies of broad interest to readers of TAAP that would benefit from rapid publication. These articles should contain no more than a combined total of four figures and tables. Authors should include in their cover letter the justification for consideration of their manuscript as a short article.