Glucan-rich polysaccharides obtained from split gill mushroom [Schizophyllum commune (Fr.)] ameliorate hyperglycemia by enhancing insulin and GLUT2 pancreas in type 2 diabetic rats.

IF 2.5 4区生物学 Q3 CELL BIOLOGY

Histology and histopathology Pub Date : 2024-06-24 DOI:10.14670/HH-18-784

Udomlak Matsathit, Decha Sermwittayawong, Manaras Komolgriengkrai, Wipapan Khimmaktong

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Abstract

Aims: Abnormalities in the secretion of insulin are the cause of pathology and complications in diabetic patients. The aim of this study was to investigate the anti-diabetic effect of polysaccharide extracts from the split gill mushroom in type 2 diabetes rats administered a low dose of streptozotocin (STZ) in combination with a high-fat diet.

Methods: The rats were divided into 6 groups: the control group (ND), the control group fed with polysaccharide extract from split gill (ND240), the diabetes group (HFD+DM), the diabetic group fed 120 (HFD+S120) and 240 mg/kg BW polysaccharide extract (HFD+S240), and the diabetic group receiving metformin (HFD+Met). Subsequently, the Islets of Langerhans of pancreatic tissue were studied using a light microscope and transmission electron microscopy (TEM). Immunofluorescence for the detection of insulin and glucose transporter 2 (GLUT2) proteins, and malondialdehyde (MDA) were also detected in pancreatic tissue.

Results: In the diabetic and HFD+120 groups, the tissues harbored various pathologies. The HFD+S240 and HFD+Met groups were found to have lower blood sugar levels. The levels of insulin and GLUT2 increased compared with the diabetic group. Additionally, the levels of MDA were reduced.

Conclusions: The use of polysaccharide extract from split gill mushrooms (240 mg/kg BW) is an alternative to treating various pathologies in the relief or treatment of diabetes mellitus.

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从裂鳃蘑菇[Schizophyllum commune (Fr.)] 中提取的富含葡聚糖的多糖可通过增强胰岛素和 GLUT2 胰腺来改善 2 型糖尿病大鼠的高血糖症状。

目的：胰岛素分泌异常是糖尿病患者出现病变和并发症的原因。本研究的目的是探究从裂鳃蘑菇中提取的多糖对给予低剂量链脲佐菌素（STZ）和高脂肪饮食的 2 型糖尿病大鼠的抗糖尿病作用：方法：将大鼠分为6组：对照组（ND）、喂食裂鳃菇多糖提取物的对照组（ND240）、糖尿病组（HFD+DM）、喂食120毫克/千克体重（HFD+S120）和240毫克/千克体重多糖提取物的糖尿病组（HFD+S240）以及服用二甲双胍的糖尿病组（HFD+Met）。随后，使用光学显微镜和透射电子显微镜（TEM）对胰腺组织的朗格汉斯胰岛进行了研究。免疫荧光法检测了胰腺组织中的胰岛素和葡萄糖转运体 2（GLUT2）蛋白以及丙二醛（MDA）：结果：糖尿病组和 HFD+120 组的胰腺组织出现了各种病变。HFD+S240组和HFD+Met组的血糖水平较低。与糖尿病组相比，胰岛素和 GLUT2 的水平升高。此外，MDA 水平也有所降低：结论：使用双孢蘑菇多糖提取物（240 毫克/千克体重）可替代治疗各种病症，缓解或治疗糖尿病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Histology and histopathology 生物-病理学

CiteScore

3.90

自引率

0.00%

发文量

232

审稿时长

2 months

期刊介绍： HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.