The effectiveness of endolysin ENDO-1252 from Salmonella bacteriophage-1252 against nontyphoidal Salmonella enterica.

IF 2.2 4区生物学 Q3 MICROBIOLOGY

Fems Microbiology Letters Pub Date : 2024-01-09 DOI:10.1093/femsle/fnae051

Chuan-Wei Tung, Dita Julianingsih, Christa Canagarajah, George Sellers, Aaron Scriba, Zabdiel Alvarado-Martínez, Zajeba Tabashsum, Debabrata Biswas

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引用次数: 0

Abstract

Salmonella enterica (S. enterica) is the most common food and waterborne pathogen worldwide. The growing trend of antibiotic-resistant S. enterica poses severe healthcare threats. As an alternative antimicrobial agent, bacteriophage-encoded endolysins (endolysins) are a potential agent in controlling S. enterica infection. Endolysins are enzymes that particularly target the peptidoglycan layer of bacterial cells, leading to their rupture and destruction. However, the application of endolysins against Gram-negative bacteria is limited due to the presence of the outer membrane in the cell wall, which hinders the permeation of externally applied endolysins. This study aimed the prokaryotic expression system to produce the recombinant endolysin ENDO-1252, encoded by the Salmonella bacteriophage-1252 associated with S. Enteritidis. Subsequently, ENDO-1252 had strong lytic activity not only against S. Enteritidis but also against S. Typhimurium. In addition, ENDO-1252 showed optimal thermostability and lytic activity at 25°C with a pH of 7.0. In combination with 0.1 mM EDTA, the effect of 120 µg of ENDO-1252 for 6 hours exhibited the highest lytic activity, resulting in a reduction of 1.15 log or 92.87% on S. Enteritidis. These findings suggest that ENDO-1252 can be used as a potential and innovative antibacterial agent for controlling the growth of S. Enteritidis.

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噬菌体沙门氏菌内溶素ENDO-1252对非伤寒沙门氏菌肠炎的有效性。

肠炎沙门氏菌（S. enterica）是全球最常见的食物和水传播病原体。肠炎沙门氏菌对抗生素的耐药性呈增长趋势，对医疗保健构成严重威胁。作为一种替代抗菌剂，噬菌体编码的内溶酶（内溶酶）是控制肠炎杆菌感染的潜在药物。内溶素是一种酶，特别针对细菌细胞的肽聚糖层，导致其破裂和破坏。然而，噬菌体编码的内溶酶在对付革兰氏阴性菌方面的应用受到限制，原因是细胞壁中存在外膜，阻碍了外部应用的内溶酶的渗透。本研究旨在利用原核表达系统生产重组内溶素ENDO-1252，该内溶素由与肠炎沙门氏菌相关的噬菌体-1252编码。随后，ENDO-1252 不仅对肠炎沙门氏菌，而且对秋伤寒沙门氏菌都具有很强的溶菌活性。此外，ENDO-1252 在 25°C、pH 值为 7.0 时显示出最佳的恒温性和溶菌活性。将 120 µg 的ENDO-1252与 0.1 mM 乙二胺四乙酸结合使用 6 小时后，ENDO-1252 的溶菌活性最高，可使肠炎双球菌的数量减少 1.15 log 或 92.87%。这些研究结果表明，ENDO-1252 可作为一种潜在的创新抗菌剂用于控制肠炎双球菌的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fems Microbiology Letters 生物-微生物学

CiteScore

4.30

自引率

0.00%

发文量

112

审稿时长

1.9 months

期刊介绍： FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.