Detection of multiple Begomoviruses in chilli crop in the five agro-climatic zones of Tamil Nadu state of India

IF 2.5 3区 农林科学 Q2 Agricultural and Biological Sciences
P. Jayanthi, Pradeep Kumar, Anirban Roy, Bikash Mandal, A. Swapna Geetanjali
{"title":"Detection of multiple Begomoviruses in chilli crop in the five agro-climatic zones of Tamil Nadu state of India","authors":"P. Jayanthi, Pradeep Kumar, Anirban Roy, Bikash Mandal, A. Swapna Geetanjali","doi":"10.1007/s40858-024-00659-z","DOIUrl":null,"url":null,"abstract":"<p>Chilli, an important vegetable cum spice crop in India, is affected by various biotic and abiotic factors, which leads to a significant reduction in the growth and yield of the chilli crop. One of the most prominent biotic factors posing threat to chilli production in southern parts of India especially, in Tamil Nadu is begomoviruses (Family <i>Geminiviridae</i>). Begomoviruses are transmitted by the insect vector, whitefly (<i>Bemisia tabaci</i>) and they cause curling, yellowing, puckering, and reduction in the size of the leaf in the infected chilli plants. In this study, molecular (PCR<b>)</b> diagnostics were used to detect the presence of begomovirus, betasatellite, and six begomovirus species viz tomato leaf curl New Delhi virus (ToLCNDV), tomato leaf curl Bangalore virus (ToLCBV), tomato leaf curl Palampur virus (ToLCPalV), tomato leaf curl Gujarat virus (ToLCGV), tomato leaf curl Joydebpur virus (ToLCJoV) and chilli leaf curl virus (ChiLCV), in the chilli samples collected from the major chilli growing areas of all the five agro-climatic zones of Tamil Nadu state of India. A total number of 833 samples collected from different locations in Tamil Nadu during the period of 2018-2022 were analysed by generic as well as species-specific PCR. The PCR results of 833 samples showed positive amplification of 20.5% for generic, 13.4% for beta satellite, 42.1% for ChiLCV, 17.8% (ToLCNDV), 16.6% (ToLCGV), 6.7% (ToLCBV), 2.2% (ToLCPalV), and 0.7% for ToLCJoV specific primers respectively. The percentage of mixed infection of two or more than two begomoviruses among the total samples is 39.2. Our study has shown that the ChiLCV and multiple tomato-infecting begomoviruses were prevalent in the major chilli-growing areas of Tamil Nadu. The present study also showed that species of begomovirus infecting chilli plants in Northern and Southern India have a differential distribution.</p>","PeriodicalId":23354,"journal":{"name":"Tropical Plant Pathology","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s40858-024-00659-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0

Abstract

Chilli, an important vegetable cum spice crop in India, is affected by various biotic and abiotic factors, which leads to a significant reduction in the growth and yield of the chilli crop. One of the most prominent biotic factors posing threat to chilli production in southern parts of India especially, in Tamil Nadu is begomoviruses (Family Geminiviridae). Begomoviruses are transmitted by the insect vector, whitefly (Bemisia tabaci) and they cause curling, yellowing, puckering, and reduction in the size of the leaf in the infected chilli plants. In this study, molecular (PCR) diagnostics were used to detect the presence of begomovirus, betasatellite, and six begomovirus species viz tomato leaf curl New Delhi virus (ToLCNDV), tomato leaf curl Bangalore virus (ToLCBV), tomato leaf curl Palampur virus (ToLCPalV), tomato leaf curl Gujarat virus (ToLCGV), tomato leaf curl Joydebpur virus (ToLCJoV) and chilli leaf curl virus (ChiLCV), in the chilli samples collected from the major chilli growing areas of all the five agro-climatic zones of Tamil Nadu state of India. A total number of 833 samples collected from different locations in Tamil Nadu during the period of 2018-2022 were analysed by generic as well as species-specific PCR. The PCR results of 833 samples showed positive amplification of 20.5% for generic, 13.4% for beta satellite, 42.1% for ChiLCV, 17.8% (ToLCNDV), 16.6% (ToLCGV), 6.7% (ToLCBV), 2.2% (ToLCPalV), and 0.7% for ToLCJoV specific primers respectively. The percentage of mixed infection of two or more than two begomoviruses among the total samples is 39.2. Our study has shown that the ChiLCV and multiple tomato-infecting begomoviruses were prevalent in the major chilli-growing areas of Tamil Nadu. The present study also showed that species of begomovirus infecting chilli plants in Northern and Southern India have a differential distribution.

Abstract Image

在印度泰米尔纳德邦五个农业气候区的辣椒作物中检测到多种贝戈莫病毒
辣椒是印度重要的蔬菜和香料作物,受到各种生物和非生物因素的影响,导致辣椒作物的生长和产量显著下降。对印度南部地区(尤其是泰米尔纳德邦)辣椒生产构成威胁的最主要生物因素之一是乞蛾病毒(Geminiviridae 科)。乞猴病毒由昆虫媒介粉虱(Bemisia tabaci)传播,它们会导致受感染的辣椒植株叶片卷曲、变黄、起皱和变小。本研究采用分子(PCR)诊断方法检测了乞乞科病毒、β卫星和六种乞乞科病毒的存在,即番茄卷叶新德里病毒(ToLCNDV)、番茄卷叶班加罗尔病毒(ToLCBV)、番茄卷叶巴兰普尔病毒(ToLCPalV)、番茄卷叶古吉拉特病毒(ToLCPalV)、番茄卷叶班加罗尔病毒(ToLCBV)、番茄卷叶巴兰普尔病毒(ToLCPalV)、番茄卷叶古吉拉特病毒(ToLCGV)、番茄卷叶乔伊德布尔病毒(ToLCJoV)和辣椒卷叶病毒(ChiLCV)。在 2018-2022 年期间从泰米尔纳德邦不同地点收集的 833 份样本均通过通用和物种特异性 PCR 进行了分析。833 份样本的 PCR 结果显示,通用引物的阳性扩增率为 20.5%,β-卫星引物为 13.4%,ChiLCV 为 42.1%,ToLCNDV 为 17.8%,ToLCGV 为 16.6%,ToLCBV 为 6.7%,ToLCCPalV 为 2.2%,ToLCJoV 特异引物为 0.7%。在所有样本中,两种或两种以上乞猴病毒混合感染的比例为 39.2%。我们的研究表明,ChiLCV 和多种番茄感染乞猴病毒在泰米尔纳德邦的主要辣椒种植区很普遍。本研究还表明,在印度北部和南部,感染辣椒植株的茄科病毒种类分布不同。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Tropical Plant Pathology
Tropical Plant Pathology PLANT SCIENCES-
CiteScore
4.50
自引率
4.00%
发文量
61
审稿时长
6-12 weeks
期刊介绍: Tropical Plant Pathology is an international journal devoted to publishing a wide range of research on fundamental and applied aspects of plant diseases of concern to agricultural, forest and ornamental crops from tropical and subtropical environments.  Submissions must report original research that provides new insights into the etiology and epidemiology of plant disease as well as population biology of plant pathogens, host-pathogen interactions, physiological and molecular plant pathology, and strategies to promote crop protection. The journal considers for publication: original articles, short communications, reviews and letters to the editor. For more details please check the submission guidelines. Founded in 1976, the journal is the official publication of the Brazilian Phytopathology Society.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信