Volume electron microscopy for genetically and molecularly defined neural circuits.

IF 2.4 4区 医学 Q3 NEUROSCIENCES
Nobuhiko Ohno, Fuyuki Karube, Fumino Fujiyama
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引用次数: 0

Abstract

The brain networks responsible for adaptive behavioral changes are based on the physical connections between neurons. Light and electron microscopy have long been used to study neural projections and the physical connections between neurons. Volume electron microscopy has recently expanded its scale of analysis due to methodological advances, resulting in complete wiring maps of neurites in a large volume of brain tissues and even entire nervous systems in a growing number of species. However, structural approaches frequently suffer from inherent limitations in which elements in images are identified solely by morphological criteria. Recently, an increasing number of tools and technologies have been developed to characterize cells and cellular components in the context of molecules and gene expression. These advancements include newly developed probes for visualization in electron microscopic images as well as correlative integration methods for the same elements across multiple microscopic modalities. Such approaches advance our understanding of interactions between specific neurons and circuits and may help to elucidate novel aspects of the basal ganglia network involving dopamine neurons. These advancements are expected to reveal mechanisms for processing adaptive changes in specific neural circuits that modulate brain functions.

用于基因和分子定义神经回路的体电子显微镜。
负责适应性行为变化的大脑网络基于神经元之间的物理连接。长期以来,人们一直使用光学显微镜和电子显微镜来研究神经投射和神经元之间的物理连接。最近,由于方法学的进步,体层电子显微镜扩大了其分析范围,在越来越多的物种中,大量脑组织甚至整个神经系统中都出现了神经元的完整布线图。然而,结构方法经常受到固有局限性的影响,即仅根据形态学标准识别图像中的元素。最近,越来越多的工具和技术被开发出来,用于根据分子和基因表达来描述细胞和细胞成分的特征。这些进步包括新开发的用于电子显微镜图像可视化的探针,以及在多种显微模式下对相同元素进行相关整合的方法。这些方法有助于加深我们对特定神经元和回路之间相互作用的理解,并有助于阐明基底神经节网络中涉及多巴胺神经元的新方面。这些进展有望揭示调节大脑功能的特定神经回路适应性变化的处理机制。
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来源期刊
Neuroscience Research
Neuroscience Research 医学-神经科学
CiteScore
5.60
自引率
3.40%
发文量
136
审稿时长
28 days
期刊介绍: The international journal publishing original full-length research articles, short communications, technical notes, and reviews on all aspects of neuroscience Neuroscience Research is an international journal for high quality articles in all branches of neuroscience, from the molecular to the behavioral levels. The journal is published in collaboration with the Japan Neuroscience Society and is open to all contributors in the world.
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